Influence of pH and pKa values on electrophoretic behaviour of quinolones in aqueous and hydro-organic media

被引：55

作者：

Barrón, D ^{[1
]}

Jiménez-Lozano, E ^{[1
]}

Irles, A ^{[1
]}

Barbosa, J ^{[1
]}

机构：

[1] Univ Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 871卷 / 1-2期

关键词：

activity coefficients; pH effects; electrophoretic mobility; dissociation constants; quinolones; antibacterial agents;

D O I：

10.1016/S0021-9673(99)01019-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Through correct pH measurements, pK(a) and activity coefficient values, a model describing their effect on electrophoretic behaviour of substances is established. The suggested model uses the pH values in the acetonitrile-water mixtures used and takes into account the effect of activity coefficients. The model permits the calculation of acidity constants of analytes in hydro-organic media and also the prediction of the effect of pH on the electrophoretic mobility. The model is tested by determining the dissociation constants of a series of nine quinolones in acetonitrile-water mixtures of 0, 5.5, 10 and 30% (w/w) acetonitrile. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：381 / 389

页数：9

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