Existence of two molecular species of collagen in the muscle layer of the ascidian (Halocynthia roretzi)

被引：21

作者：

Mizuta, S ^{[1
]}

Isobe, S ^{[1
]}

Yoshinaka, R ^{[1
]}

机构：

[1] Fukui Prefectural Univ, Dept Marine Biosci, Fac Biotechnol, Obama, Fukui 9170003, Japan

来源：

FOOD CHEMISTRY | 2002年 / 79卷 / 01期

关键词：

collagen; sea squirt; ascidian; muscle; connective tissue;

D O I：

10.1016/S0308-8146(02)00167-X

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The molecular species of collagen in the muscle layer of the ascidian Halocynthia roretzi was examined by biochemical techniques. Two types of collagen which showed distinct patterns from each other on SDS-PAGE were isolated from the pepsin-solubilized collagen by differential salt precipitation and phosphocellulose column chromatography. They were demonstrated to be genetically distinct from each other by peptide mapping and by amino acid analysis. These results indicate that at least two molecular species of collagen are present in the muscle layer of the ascidian. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：9 / 13

页数：5

共 26 条

[1] POSTMORTEM CHANGE OF 3-DIMENSIONAL STRUCTURE OF COLLAGEN FIBRILLAR NETWORK IN FISH MUSCLE PERICELLULAR CONNECTIVE TISSUES CORRESPONDING TO POSTMORTEM TENDERIZATION [J].

ANDO, M ;

YOSHIMOTO, Y ;

INABU, K ;

NAKAGAWA, T ;

MAKINODAN, Y .

FISHERIES SCIENCE, 1995, 61 (02) :327-330

[2]

CLEVELAND DW, 1977, J BIOL CHEM, V252, P1102

[3]

DUHAMEL RC, 1983, COLLAGEN REL RES, V3, P195

[4] ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].

FAIRBANKS, G ;

STECK, TL ;

WALLACH, DFH .

BIOCHEMISTRY, 1971, 10 (13) :2606-+

[5]

KIMURA S, 1983, B JPN SOC SCI FISH, V49, P229

[6] CHARACTERIZATION OF FISH MUSCLE TYPE-I COLLAGEN [J].

KIMURA, S ;

ZHU, XP ;

MATSUI, R ;

SHIJOH, M ;

TAKAMIZAWA, S .

JOURNAL OF FOOD SCIENCE, 1988, 53 (05) :1315-1318

[7]

KIMURA S, 1968, BULL JAP SOC SCI FISH, V34, P925

[8]

KIMURA S, 1972, B JPN SOC SCI FISH, V38, P1163

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] METACHROMASY OF PERIPHERAL-NERVE COLLAGEN ON POLYACRYLAMIDE GELS STAINED WITH COOMASSIE BRILLIANT BLUE R-250 [J].

MICKO, S ;

SCHLAEPFER, WW .

ANALYTICAL BIOCHEMISTRY, 1978, 88 (02) :566-572

← 1 2 3 →