Identification and verification of novel rodent postsynaptic density proteins

被引:238
作者
Jordan, BA
Fernholz, BD
Boussac, M
Xu, CF
Grigorean, G
Ziff, EB
Neubert, TA [1 ]
机构
[1] NYU, Sch Med, Skirball Inst Biomol Med Lab 5 18, New York, NY 10016 USA
[2] NYU, Sch Med, Dept Biochem, New York, NY 10016 USA
[3] NYU, Sch Med, Dept Physiol & Neurosci, New York, NY 10016 USA
[4] NYU, Sch Med, Dept Pharmacol, New York, NY 10016 USA
关键词
D O I
10.1074/mcp.M400045-MCP200
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The postsynaptic density (PSD) is a cellular structure specialized in receiving and transducing synaptic information. Here we describe the identification of 452 proteins isolated from biochemically purified PSD fractions of rat and mouse brains using nanoflow HPLC coupled to electrospray tandem mass spectrometry (LC-MS/MS). Fluorescence microscopy and Western blotting were used to verify that many of the novel proteins identified exhibit subcellular distributions consistent with those of PSD-localized proteins. In addition to identifying most previously described PSD components, we also detected proteins involved in signaling to the nucleus as well as regulators of ADP-ribosylation factor signaling, ubiquitination, RNA trafficking, and protein translation. These results suggest new mechanisms by which the PSD helps regulate synaptic strength and transmission.
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收藏
页码:857 / 871
页数:15
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