Altered phosphofructokinase mRNA levels but unchanged isoenzyme pattern in brains from patients with Alzheimer's disease

被引：12

作者：

Bigl, M

Beck, MM

Bleyl, AD

Bigl, V

Eschrich, K

机构：

[1] Univ Leipzig, Sch Med, Inst Biochem, D-04103 Leipzig, Germany

[2] Univ Leipzig, Sch Med, Paul Flechsig Inst Brain Res, Dept Neurochem, D-04103 Leipzig, Germany

来源：

MOLECULAR BRAIN RESEARCH | 2000年 / 76卷 / 02期

关键词：

phosphofructokinase mRNA; phosphofructokinase isoenzyme; Alzheimer's disease; ribonuclease protection assay; quantitative PCR;

D O I：

10.1016/S0169-328X(00)00019-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In order to find out whether the increased phosphofructokinase (PFK) activities observed in brains from Alzheimer's disease (AD) patients are associated with alterations in PFK mRNA levels, we determined total PFK mRNA and the three different PFK isoenzyme mRNAs in AD and control patients by ribonuclease protection assay (RPA) and quantitative RT-PCR. PFK mRNA levels were found increased in some brain areas in AD patients. While all three PFK isoenzyme mRNAs were detectable in every studied brain sample, no changes of the PFK isoenzyme pattern were observed in patients with AD. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：411 / 414

页数：4

共 21 条

[1] Activities of key glycolytic enzymes in the brains of patients with Alzheimer's disease [J].