Measuring absorption coefficients in small volumes of highly scattering media: Source-detector separations for which path lengths do not depend on scattering properties

被引：126

作者：

Mourant, JR

Bigio, IJ

Jack, DA

Johnson, TM

Miller, HD

机构：

[1] Bioscience and Biotechnology Group CST-4, Los Alamos National Laboratory, Los Alamos, NM

来源：

APPLIED OPTICS | 1997年 / 36卷 / 22期

关键词：

absorbance measurements; turbid media; tissue absorption; concentration measurements;

D O I：

10.1364/AO.36.005655

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The noninvasive measurement of variations in absorption that are due to changes in concentrations of biochemically relevant compounds in tissue is important in many clinical settings. One problem with such measurements is that the path length traveled by the collected light through the tissue depends on the scattering properties of the tissue. We demonstrate, using both Monte Carlo simulations and experimental measurements, that for an appropriate separation between light-delivery and light collection fibers the path length of the collected photons does not depend on scattering parameters for the range of parameters typically found in tissue. This is important for developing rapid, noninvasive, and inexpensive methods for measuring absorption changes in tissue. (C) 1997 Optical Society of America.

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收藏

页码：5655 / 5661

页数：7

相关论文

共 15 条

[1] NEAR-INFRARED SPECTROSCOPY OF THE BRAIN - RELEVANCE TO CYTOCHROME-OXIDASE BIOENERGETICS [J].

COOPER, CE ;

MATCHER, SJ ;

WYATT, JS ;

COPE, M ;

BROWN, GC ;

NEMOTO, EM ;

DELPY, DT .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1994, 22 (04) :974-980

[2] DO ANTICANCER AGENTS REACH THE TUMOR TARGET IN THE HUMAN BRAIN [J].

DONELLI, MG ;

ZUCCHETTI, M ;

DINCALCI, M .

CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1992, 30 (04) :251-260

[3] QUANTITATIVE-DETERMINATION OF THE ABSORPTION-SPECTRA OF CHROMOPHORES IN STRONGLY SCATTERING MEDIA - A LIGHT-EMITTING-DIODE BASED TECHNIQUE [J].

FANTINI, S ;

FRANCESCHINI, MA ;

FISHKIN, JB ;

BARBIERI, B ;

GRATTON, E .

APPLIED OPTICS, 1994, 33 (22) :5204-5213

[4] A DIFFUSION-THEORY MODEL OF SPATIALLY RESOLVED, STEADY-STATE DIFFUSE REFLECTANCE FOR THE NONINVASIVE DETERMINATION OF TISSUE OPTICAL-PROPERTIES INVIVO [J].

FARRELL, TJ ;

PATTERSON, MS ;

WILSON, B .

MEDICAL PHYSICS, 1992, 19 (04) :879-888

[5]

KERR DJ, 1993, CANCER SURV, V17, P105

[6] Spatially resolved absolute diffuse reflectance measurements for noninvasive determination of the optical scattering and absorption coefficients of biological tissue [J].

Kienle, A ;

Lilge, L ;

Patterson, MS ;

Hibst, R ;

Steiner, R ;

Wilson, BC .

APPLIED OPTICS, 1996, 35 (13) :2304-2314

[7] DETERMINATION OF REDUCED SCATTERING AND ABSORPTION-COEFFICIENTS BY A SINGLE CHARGE-COUPLED-DEVICE ARRAY MEASUREMENT .1. COMPARISON BETWEEN EXPERIMENTS AND SIMULATIONS [J].

MARQUET, P ;

BEVILACQUA, F ;

DEPEURSINGE, C ;

DEHALLER, EB .

OPTICAL ENGINEERING, 1995, 34 (07) :2055-2063

[8] Influence of the scattering phase function on light transport measurements in turbid media performed with small source-detector separations [J].

Mourant, JR ;

Boyer, J ;

Hielscher, AH ;

Bigio, IJ .

OPTICS LETTERS, 1996, 21 (07) :546-548

[9] Predictions and measurements of scattering and absorption over broad wavelength ranges in tissue phantoms [J].

Mourant, JR ;

Fuselier, T ;

Boyer, J ;

Johnson, TM ;

Bigio, IJ .

APPLIED OPTICS, 1997, 36 (04) :949-957

[10] THE MEASUREMENT OF DIHEMATOPORPHYRIN ETHER CONCENTRATION IN TISSUE BY REFLECTANCE SPECTROPHOTOMETRY [J].

PATTERSON, MS ;

WILSON, BC ;

FEATHER, JW ;

BURNS, DM ;

PUSHKA, W .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1987, 46 (03) :337-343