The selection of ELISA cut-off points for testing antibody to Newcastle disease by two-graph receiver operating characteristic (TG-ROC) analysis

被引：24

作者：

Xu, H

Lohr, J

Greiner, M

机构：

[1] STAATLICHES TIERARZTLICHES UNTERSUCHUNGSAMT AULEN,D-88326 AULENDORF,GERMANY

[2] ZHEJIANG ACAD AGR SCI,VIROL LAB,HANGZHOU 310021,PEOPLES R CHINA

[3] FREE UNIV BERLIN,INST PARASITOL & TROP VET MED,D-14163 BERLIN,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1997年 / 208卷 / 01期

关键词：

ND; ELISA; HI rest; cut-off point; two-graph receiver operating characteristic(TG-ROC) analysis; sensitivity and specificity; efficiency; Youden's index; virology;

D O I：

10.1016/S0022-1759(97)00128-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two hundred and sixty field serum samples were tested for Newcastle disease (ND) antibodies using a commercial enzyme-linked immunosorbent assay (ELISA) and the haemagglutination inhibition test (HI). The HI test was regarded as the reference method. Reciprocal titres of 16 and above were considered positive. In this study the two-graph receiver operating characteristic (TG-ROC) analysis was used as a tool for selecting cut-off points. Sensitivity, specificity, efficiency and Youden's index were used as indices of test accuracy. The positive and negative predictive values of the ELISA results were analysed for various prevalence rates. (C) 1997 Elsevier Science B.V.

引用

页码：61 / 64

页数：4

共 6 条

[1]

Allan W H., 1978, Newcastle disease vaccines-Their production and use. FAO Animal Production and Health Series No. 10

[2]

ARMITAGE P, 1987, STATISTICAL METHODS, P472

[3]

Galen RS, 1986, MANUAL CLIN LAB IMMU, V3rd, P966

[4] 2-GRAPH RECEIVER OPERATING CHARACTERISTIC (TG-ROC) - A MICROSOFT-EXCEL TEMPLATE FOR THE SELECTION OF CUTOFF VALUES IN DIAGNOSTIC-TESTS [J].