Photocontrol of subcellular partitioning of phytochrome-B:GFP fusion protein in tobacco seedlings

被引:51
作者
Gil, P
Kircher, S
Adam, E
Bury, E
Kozma-Bognar, L
Schäfer, E
Nagy, F
机构
[1] Inst Plant Biol, Biol Res Ctr, H-6701 Szeged, Hungary
[2] Univ Freiburg, Inst Biol 2, D-79104 Freiburg, Germany
关键词
D O I
10.1046/j.1365-313x.2000.00730.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Photomorphogenesis of higher plants is regulated by photoreceptors including the red/far-red light-absorbing phytochromes, blue-UV/A sensing cryptochromes and as yet uncharacterized UV/B receptors. Specific phototransduction pathways that are controlled by either individual or interacting photoreceptors mediate regulation. Phytochrome B (phyB) is the major red light-sensing photoreceptor. Phototransduction mediated by this light sensor has been shown to include light-dependent nuclear import and interaction of phyB with transcription factor-like proteins in the nucleus. Here we report that nuclear import of phyB and physiological responses regulated by this photoreceptor exhibit very similar wavelength- and fluence rate-dependence. Nuclear import of phyB is insensitive to single red, blue and far-red light pulses. It is induced by continuous red light and to a lesser extent by continuous blue light, whereas far-red light is completely ineffective. The data presented indicate that light-dependent partitioning of phyB exhibits features characteristic of blue light responsiveness amplification, a phenomenon that is thought to be mediated by interaction of phyB with CRY1.
引用
收藏
页码:135 / 145
页数:11
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