Characterization of enterotoxigenic Escherichia coli by random amplification of polymorphic DNA

被引：34

作者：

Pacheco, ABF

Guth, BEC

deAlmeida, DF

Ferreira, LCS

机构：

[1] UNIV FED RIO DE JANEIRO,CCS,INST BIOFIS CARLOS CHAGAS FILHO,BR-21941590 RIO JANEIRO,BRAZIL

[2] ESCOLA PAULISTA MED,DISCIPLINA MICROBIOL & IMUNOL,BR-04023062 SAO PAULO,BRAZIL

来源：

RESEARCH IN MICROBIOLOGY | 1996年 / 147卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

ETEC; enteritis; Escherichia coli; RAPD; epidemiological tool; genetic polymorphism;

D O I：

10.1016/0923-2508(96)80217-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two enterotoxigenic Escherichia coli (ETEC) strains (H10407 and 4011-1) were characterized by random amplification of polymorphic DNA (RAPD) profiles using 10-mer oligonucleotides with diverse GC content, All tested primers yielded arrays of amplified DNA products ranging in size from 200 to 3000 bp. The effects of annealing temperature, template concentration and GC content of the primers were evaluated and an optimal reaction procedure was established. Application of the RAPD analysis to ten ETEC strains belonging to five different serotypes showed that strains of the same serotype shared identical or almost identical band profiles, suggesting a similar genetic composition. The use of RAPD profiles as a tool in epidemiological analysis of ETEC is discussed.

引用

页码：175 / 182

页数：8

共 37 条

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