A new perfusion cell chamber system for determination of heat shock effects by means of video-enhanced microscopy

被引：9

作者：

Hofer, A ^{[1
]}

Nagel, F ^{[1
]}

Wonka, F ^{[1
]}

Krinke, HE ^{[1
]}

Gölfert, F ^{[1
]}

Funk, RHW ^{[1
]}

机构：

[1] Tech Univ Dresden, Inst Anat, D-01307 Dresden, Germany

来源：

MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING | 1999年 / 37卷 / 05期

关键词：

perfusion chamber; monitoring; video-enhanced contrast microscopy (VECM); heat shock; mitochondria; astrocytes;

D O I：

10.1007/BF02513364

中图分类号：

TP39 [计算机的应用];

学科分类号：

081203 ; 0835 ;

摘要：

A user friendly microscope perfusion chamber which allows real-time observation of individual cells at high magnification has been designed. An integrated multisensor was used to monitor the cell culture conditions. To prove the potential of the system heat shock experiments were performed By means of video-enhanced contrast microscopy (VECM) the mitochondria morphology of cultured astrocytes was demonstrated to change from a rod-like to an annular shape after heat shock. For further analyses mitochondria were stained on the microscope stage.

引用

页码：667 / 669

页数：3

共 15 条

[1]

Besch SR, 1996, UNDERSEA HYPERBAR M, V23, P175

[2] VERSATILE PERFUSION CHAMBER FOR HIGH-MAGNIFICATION MICROSCOPY [J].