Production of a monoclonal antibody directed against the recombinant SEL1L protein

被引：18

作者：

Orlandi, R

Cattaneo, A

Troglio, F

Campiglio, M

Biunno, I

Ménard, S

机构：

[1] Ist Nazl Tumori, Dept Expt Oncol, Mol Targeting Unit, I-20133 Milan, Italy

[2] CNR, Inst Biomed Technol, Segrate, Milan, Italy

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS | 2002年 / 17卷 / 02期

关键词：

SEL1L; monoclonal antibody; lung carcinoma;

D O I：

10.1177/172460080201700205

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

SEL1L, highly similar to the C. elegans sel-1 gene, is a recently cloned human gene whose function is under investigation. SEL1L is differentially expressed in tumors and normal tissues and seems to play a role in tumor growth and aggressiveness. We used the recombinant N-terminus of the SEL1L protein to immunize a Balb/c mouse and produce a monoclonal antibody. A hybridoma secreting an antibody specifically reacting on the SEL1L recombinant fragment was selected. This monoclonal antibody, named MSel1, recognizes the SEL1L protein by Western blotting, immunofluorescence and immunohistochemistry on normal and tumor cells. MSel1 is able to recognize SEL1L even on archival tumor specimens and is therefore particularly appropriate to study SEL1L involvement in tumor progression.

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页码：104 / 111

页数：8

相关论文

共 16 条

[1] Notch signaling: Cell fate control and signal integration in development [J].

Artavanis-Tsakonas, S ;

Rand, MD ;

Lake, RJ .

SCIENCE, 1999, 284 (5415) :770-776

[2] SEL1L microsatellite polymorphism in Japanese patients with autoimmune thyroid diseases [J].

Ban, Y ;

Taniyama, M ;

Tozaki, T ;

Yanagawa, T ;

Tomita, M ;

Ban, Y .

THYROID, 2001, 11 (04) :335-338

[3] SEL1L, the human homolog of C-elegans sel-1:: refined physical mapping, gene structure and identification of polymorphic markers [J].

Biunno, I ;

Bernard, L ;

Dear, P ;

Cattaneo, M ;

Volorio, S ;

Zannini, L ;

Bankier, A ;

Zollo, M .

HUMAN GENETICS, 2000, 106 (02) :227-235

[4] Isolation of a pancreas-specific gene located on human chromosome 14q31: Expression analysis in human pancreatic ductal carcinomas [J].

Biunno, I ;

Appierto, V ;

Cattaneo, M ;

Leone, BE ;

Balzano, G ;

Socci, C ;

Saccone, S ;

Letizia, A ;

DellaValle, G ;

Sgaramella, V .

GENOMICS, 1997, 46 (02) :284-286

[5] The expression of SEL1L and TAN-1 in normal and neoplastic cells [J].

Cattaneo, M ;

Orlandi, R ;

Ronchini, C ;

Granelli, P ;

Malferrari, G ;

Menard, S ;

Biunno, I .

INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS, 2000, 15 (01) :26-32

[6] ANTIGEN UNMASKING ON FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE-SECTIONS [J].

CATTORETTI, G ;

PILERI, S ;

PARRAVICINI, C ;

BECKER, MHG ;

POGGI, S ;

BIFULCO, C ;

KEY, G ;

DAMATO, L ;

SABATTINI, E ;

FEUDALE, E ;

REYNOLDS, F ;

GERDES, J ;

RILKE, F .

JOURNAL OF PATHOLOGY, 1993, 171 (02) :83-98

[7] Cloning and characterization of Sel-11, a murine homolog of the C-elegans sel-1 gene [J].

Donoviel, DB ;

Donoviel, MS ;

Fan, E ;

Hadjantonakis, AK ;

Bernstein, A .

MECHANISMS OF DEVELOPMENT, 1998, 78 (1-2) :203-207

[8] SEL-1L maps to human chromosome 14, near the insulin-dependent diabetes mellitus locus 11 [J].

Donoviel, DB ;

Bernstein, A .

GENOMICS, 1999, 56 (02) :232-233

[9] Susceptibility to insulin-dependent diabetes mellitus maps to a locus (IDDM11) on human chromosome 14q24.3-q31 [J].

Field, LL ;

Tobias, R ;

Thomson, G ;

Plon, S .

GENOMICS, 1996, 33 (01) :1-8

[10]

Grant B, 1997, DEVELOPMENT, V124, P637