Metabolites of the biocide o-phenylphenol generate oxidative DNA lesions in V 79 cells

被引：12

作者：

Henschke, P ^{[1
]}

Almstadt, E ^{[1
]}

Lüttgert, S ^{[1
]}

Appel, KE ^{[1
]}

机构：

[1] Fed Inst Hlth Protect Consumers & Vet Med, Dept Plant Protect Prod & Pesticides, D-14191 Berlin, Germany

来源：

ARCHIVES OF TOXICOLOGY | 2000年 / 73卷 / 10-11期

关键词：

ortho-phenylphenol; phenylhydro-quinone; phenylbenzoquinone; carcinogenicity; oxidative DNA damage;

D O I：

10.1007/s002040050014

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Incubation of the o-phenylphenol (OPP) metabolites, o-phenylhydroquinone (PHQ) and o-phenylbenzoquitlone (PBQ) with V 79 Chinese hamster cells led to a significant enhancement of the amount of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in nuclear DNA. With OPP no distinct induction of this lesion could be observed. In addition, PHQ and PBQ were able to generate DNA single-strand breaks (DNA SSB), while OPP failed to induce this lesion. All incubations were performed for 1 h without exogenous metabolic activations and the lowest effective concentration tested was 20 mu M. It is concluded that these metabolites may contribute to the carcinogenicity of OPP and sodium o-phenylphenolate (SOPP) observed in rats, by generating reactive oxygen species (ROS) through their redox cycling properties.

引用

页码：607 / 610

页数：4

共 26 条

[1] CHEMICAL CANCEROGENESIS - DEFINITIONS OF FREQUENTLY USED TERMS [J].