Histamine induces melanogenesis and morphologic changes by protein kinase A activation via H2 receptors in human normal melanocytes

被引:108
作者
Yoshida, M [1 ]
Takahashi, Y [1 ]
Inoue, S [1 ]
机构
[1] Kanebo Ltd, Basic Res Lab, Odawara, Kanagawa, Japan
关键词
cyclic AMP; famotidine; pigmentation; tyrosinase;
D O I
10.1046/j.1523-1747.2000.00874.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Hyperpigmentation frequently accompanies chronic or acute inflammation. A number of inflammatory mediators have been shown to stimulate melanin synthesis in human melanocytes. Although histamine is ubiquitous as an inflammatory factor, its involvement in pigmentation remains obscure. In this work, we examined the effects of histamine on cultured human melanocytes, Treatment of human melanocytes with 0.1-10 mu M histamine evoked morphologic changes and increases in tyrosinase activity. The concomitant increases in melanin content of the histamine-treated melanocytes indicated an elevation of melanin synthesis by tyrosinase activation. These stimulatory effects of histamine mere completely inhibited by an H-2 antagonist, famotidine, whereas H-1 and H-3 antagonists had no inhibitory effect whatsoever, In addition, an H-2 agonist, dimaprit, induced the same degree of melanogenesis as histamine at concentrations of 0.1-10 mu M. We observed an increase in the intracellular cAMP contents of human melanocytes induced by histamine via the H-2 receptors, We know that this cAMP accumulation and subsequent protein kinase A activation plays a critical role in histamine-induced melanogenesis, because a specific protein kinase A inhibitor, H-89, completely suppressed these stimulatory effects of histamine, and because dibutylic cAMP, a specific protein kinase A activator, stimulated human melanocytes as potently as histamine. Taken together, we show here that histamine induces melanogenesis of human cultured melanocytes by protein kinase A activation via H-2 receptors.
引用
收藏
页码:334 / 342
页数:9
相关论文
共 29 条
[1]  
ARRANG JM, 1994, CELL MOL BIOL, V40, P273
[2]   BLEOMYCIN ENHANCES THE TYROSINASE ACTIVITY OF HUMAN-MALIGNANT MELANOMA-CELLS IN CULTURE [J].
BERND, A ;
ALTMEYER, P ;
SCHAFER, G ;
MARSCH, WC ;
HOLZMANN, H .
PHARMACOLOGICAL RESEARCH COMMUNICATIONS, 1986, 18 (11) :1075-1091
[3]   Effect of arbutin on melanogenic proteins in human melanocytes [J].
Chakraborty, AK ;
Funasaka, Y ;
Komoto, M ;
Ichihashi, M .
PIGMENT CELL RESEARCH, 1998, 11 (04) :206-212
[4]   MODES OF FGF RELEASE INVIVO AND INVITRO [J].
DAMORE, PA .
CANCER AND METASTASIS REVIEWS, 1990, 9 (03) :227-238
[5]   NEW TABLES FOR MULTIPLE COMPARISONS WITH CONTROL [J].
DUNNETT, CW .
BIOMETRICS, 1964, 20 (03) :482-&
[6]   DIMAPRIT ANALOGS INHIBIT TYROSINASE VIA A DISULFIDE BREAKDOWN PRODUCT INDEPENDENTLY OF THE HISTAMINE-H2-RECEPTOR [J].
FECHNER, GA ;
JACOBS, JJ ;
PARSONS, PG .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 201 (02) :687-693
[7]   THE HUMAN SUNBURN REACTION - HISTOLOGIC AND BIOCHEMICAL-STUDIES [J].
GILCHREST, BA ;
SOTER, NA ;
STOFF, JS ;
MIHM, MC .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1981, 5 (04) :411-422
[8]  
GROS GL, 1994, MELANOMA RES, V4, P359
[9]   ENDOTHELIN-1 OF KERATINOCYTE ORIGIN IS A MEDIATOR OF MELANOCYTE DENDRICITY [J].
HARA, M ;
YAAR, M ;
GILCHREST, BA .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1995, 105 (06) :744-748
[10]   ACTH STIMULATES MELANOGENESIS IN CULTURED HUMAN MELANOCYTES [J].
HUNT, G ;
TODD, C ;
KYNE, S ;
THODY, AJ .
JOURNAL OF ENDOCRINOLOGY, 1994, 140 (01) :R1-R3