Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain

被引：63

作者：

Alam, Steven L.

Langelier, Charles

Whitby, Frank G.

Koirala, Sajjan

Robinson, Howard

Hill, Christopher P. ^{[1
]}

Sundquist, Wesley I.

机构：

[1] Univ Utah, Dept Biochem, Salt Lake City, UT 84112 USA

[2] 463 Brookhaven Natl Lab, Upton, NY 11973 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2006年 / 13卷 / 11期

关键词：

D O I：

10.1038/nsmb1160

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ESCRT-I and ESCRT-II complexes help sort ubiquitinated proteins into vesicles that accumulate within multivesicular bodies (MVBs). Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the b-sandwich. The structure suggests how human ESCRT-II can couple recognition of ubiquitinated cargoes and endosomal phospholipids during MVB protein sorting.

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收藏

页码：1029 / 1030

页数：2

相关论文

共 15 条

[1] Ubiquitin interactions of NZF zinc fingers [J].

Alam, SL ;

Sun, J ;

Payne, M ;

Welch, BD ;

Blake, BK ;

Davis, DR ;

Meyer, HH ;

Emr, SD ;

Sundquist, WI .

EMBO JOURNAL, 2004, 23 (07) :1411-1421

[2]

Birkeland HCG, 2004, CURR TOP MICROBIOL, V282, P89

[3] Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains [J].

Ferguson, KM ;

Kavran, JM ;

Sankaran, VG ;

Fournier, E ;

Isakoff, SJ ;

Skolnik, EY ;

Lemmon, MA .

MOLECULAR CELL, 2000, 6 (02) :373-384

[4] Ubiquitin-binding domains [J].

Hicke, L ;

Schubert, HL ;

Hill, CP .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (08) :610-621

[5] Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting [J].

Hirano, S ;

Kawasaki, M ;

Ura, H ;

Kato, R ;

Raiborg, C ;

Stenmark, H ;

Wakatsuki, S .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 13 (03) :272-277

[6] The ESCRT complexes: Structure and mechanism of a membrane-trafficking network [J].

Hurley, James H. ;

Emr, Scott D. .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2006, 35 :277-298

[7] STRUCTURE OF THE BINDING-SITE FOR INOSITOL PHOSPHATES IN A PH DOMAIN [J].

HYVONEN, M ;

MACIAS, MJ ;

NILGES, M ;

OSCHKINAT, H ;

SARASTE, M ;

WILMANNS, M .

EMBO JOURNAL, 1995, 14 (19) :4676-4685

[8] Pleckstrin homology domains: Two halves make a hole? [J].

Lemmon, MA .

CELL, 2005, 120 (05) :574-576

[9] Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain [J].

Slagsvold, T ;

Aasland, R ;

Hirano, S ;

Bache, KG ;

Raiborg, C ;

Trambaiolo, D ;

Wakatsuki, S ;

Stenmark, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (20) :19600-19606

[10] Ubiquitin recognition by the human TSG101 protein [J].

Sundquist, WI ;

Schubert, HL ;

Kelly, BN ;

Hill, GC ;

Holton, JM ;

Hill, CP .

MOLECULAR CELL, 2004, 13 (06) :783-789