Recovery of the resurrection plant Craterostigma wilmsii from desiccation:: protection versus repair

被引:80
作者
Cooper, K
Farrant, JM
机构
[1] Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, South Africa
[2] Univ Cape Town, Dept Bot, ZA-7701 Rondebosch, South Africa
基金
新加坡国家研究基金会;
关键词
Craterostigma wilmsii; drying rate; homoiochlorophyllous; metabolic inhibitors; modified desiccation tolerance; transcription; translation;
D O I
10.1093/jxb/erf028
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Craterostigma wilmsii Engl. (homoiochlorophyllous) is a resurrection species that is thought to rely primarily on the protection of cellular components during drying to survive desiccation. The time taken for this protection to be instituted is thought to preclude recovery after rapid drying. Thus the response of C. wilmsii plants to rapid dehydration was investigated. The effect of rapid drying on sucrose accumulation was determined and the cellular ultrastructure was investigated during natural (slow) and rapid dehydration and on subsequent rehydration. The dependence of naturally and rapidly dried C. wilmsii on de novo transcription and translation during and after rehydration was determined by examining quantum efficiency, changes in photosynthetic pigments and subcellular organization of excised leaves with rehydration in water and using the metabolic inhibitors, distamycin A and cycloheximide. Slowly dried C. wilmsii required no new transcription or translation during rehydration in order to recover. With rapid dehydration, cells showed ultrastructural damage, which indicated that at least some protective mechanisms were affected (as evidenced by a reduced accumulation of sucrose). C. wilmsii was able to limit the damage and recover upon rehydration in water, but rapidly dried plants did not survive if mRNA or protein synthesis was inhibited by distamycin A or cycloheximide, respectively. This demonstrates an induction of repair mechanisms during rehydration, which enables recovery from rapid drying. Thus, although C. wilmsii does rely almost entirely on protection during natural drying, it apparently has the ability to repair if protection is inadequate and damage is incurred.
引用
收藏
页码:1805 / 1813
页数:9
相关论文
共 33 条
[1]   Light and stage of development influence the expression of desiccation-induced genes in the resurrection plant Craterostigma plantagineum [J].
Alamillo, JM ;
Bartels, D .
PLANT CELL AND ENVIRONMENT, 1996, 19 (03) :300-310
[2]  
[Anonymous], 1980, CHILLING FREEZING HI
[3]  
BARTELS D, 1990, PLANTA, V181, P27, DOI 10.1007/BF00202321
[4]  
BERGMEYER HU, 1974, METHODS ENZYMATIC AN, V2
[5]   ADAPTATIONS TO ENVIRONMENTAL STRESSES [J].
BOHNERT, HJ ;
NELSON, DE ;
JENSEN, RG .
PLANT CELL, 1995, 7 (07) :1099-1111
[6]   INTERACTIONS OF SUGARS WITH MEMBRANES [J].
CROWE, JH ;
CROWE, LM ;
CARPENTER, JF ;
RUDOLPH, AS ;
WISTROM, CA ;
SPARGO, BJ ;
ANCHORDOGUY, TJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 947 (02) :367-384
[7]   Use of metabolic inhibitors to elucidate mechanisms of recovery from desiccation stress in the resurrection plant Xerophyta humilis [J].
Dace, H ;
Sherwin, HW ;
Illing, N ;
Farrant, JM .
PLANT GROWTH REGULATION, 1998, 24 (03) :171-177
[8]   A comparison of mechanisms of desiccation tolerance among three angiosperm resurrection plant species [J].
Farrant, JM .
PLANT ECOLOGY, 2000, 151 (01) :29-39
[9]  
FARRANT JM, 1999, ANN BOT, V84, P1
[10]  
FARRANT JM, 1998, PROGR SEED RES, P109