Pseudotype hepatitis C virus enters immature myeloid dendritic cells through the interaction with lectin

被引：25

作者：

Kaimori, A

Kanto, T

Limn, CK

Komoda, Y

Oki, C

Inoue, M

Miyatake, H

Itose, I

Sakakibara, M

Yakushijin, T

Takehara, T

Matsuura, Y

Hayashi, N

机构：

[1] Osaka Univ, Grad Sch Med, Dept Mol Therapeut, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Med, Dept Dendrit Cell Biol & Clin Applicat, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Res Inst Microbial Dis, Res Ctr Emerging Infect Dis, Suita, Osaka, Japan

来源：

VIROLOGY | 2004年 / 324卷 / 01期

关键词：

hepatitis C virus; envelope proteins; pseudotype virus; dendritic cells; lectin;

D O I：

10.1016/j.virol.2004.03.038

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Dendritic cells (DC) are the most potent antigen-presenting cells that regulate immune responses. One of the mechanisms for hepatitis C virus (HCV) persistence is the ability of HCV to suppress DC function. Direct HCV infection to blood DC has been implicated for DC dysfunction. To clarify the susceptibility of each DC subset to HCV, we used pseudotype vesicular stomatitis virus (VSV) coated with chimeric HCV envelope glycoproteins (E1 and E2). We demonstrate that pseudotype VSV enters myeloid DC (MDC) but not plasmacytoid DC (PDC). The highest efficiency of pseudotype VSV entry to MDC was observed when MDC were cultured with GM-CSF. Such efficiency decreased when MDC are matured with the treatment of IL-4, CpG oligodeoxynucleotide, or CD40 ligand. Mannan inhibited pseudotype VSV entry to MDC, but Ca2+ chelators failed to do so. These results show that pseudotype VSV possessing HCV-E1 and E2 enters immature MDC through the interaction with lectins in a Ca2+-independent manner. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：74 / 83

页数：10

共 35 条

[1] DETECTION OF ANTIBODY TO HEPATITIS-C VIRUS IN PROSPECTIVELY FOLLOWED TRANSFUSION RECIPIENTS WITH ACUTE AND CHRONIC NON-A-HEPATITIS, NON-B-HEPATITIS [J].