Activation of human granulocytes by intravenous immunoglobulin preparations is mediated by FcγRII and FcγRIII receptors

被引:13
作者
Nemes, E
Teichman, F
Roos, D
Maródi, L
机构
[1] Univ Debrecen, Sch Med, Dept Infectol & Pediat Immunol, H-4012 Debrecen, Hungary
[2] Univ Debrecen, Cent Res Lab, Med & Hlth Sci Ctr, H-4012 Debrecen, Hungary
[3] Netherlands Red Cross, Blood Transfus Serv, Cent Lab, NL-1006 AD Amsterdam, Netherlands
[4] Univ Amsterdam, Acad Med Ctr, Expt & Clin Immunol Lab, NL-1006 AD Amsterdam, Netherlands
关键词
D O I
10.1203/00006450-200003000-00012
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Previous studies from our laboratory have shown that i.v. Ig (IVIG) exposure triggers superoxide anion (O-2(-)) generation by and increases bactericidal capacity of human blood granulocytes. However, the molecular interactions between IVIG and granulocytes have not been evaluated before. The objective of this study was to investigate the role of Fc gamma RII and Fc gamma RIII receptors in the immunomodulatory effects of IVIG concentrates on granulocytes. We found that four different IVIG preparations (concentration range, 1-10 mg/mL) shared the ability to stimulate O-2(-) release in vitro by granulocytes in a dose-dependent manner. Dimers fractionated from IVIG were significantly more potent in inducing activity of the respiratory burst than were monomers. MAI, (concentration range, 0.1-10 mu g/mL) specific for Fc gamma RII and Fc gamma RIII receptors inhibited the IVIG-induced 0(2)(-) release, with a more profound inhibitory effect observed with anti-Fc gamma RIII. These findings suggest the involvement of Fc gamma receptors in triggering O-2(-) release by granulocytes exposed to IVIG. We also report that IVIG added to granulocyte suspensions elicited a rapid and vigorous increase in the concentration of cytosolic free calcium, a finding suggesting direct activation and not priming of granulocytes by IVIG through Fc gamma RII and Fc gamma RIII receptors. The in vitro effects described here might occur in patients treated with IVIG and may, in part, be responsible for inflammatory reactions evoked by infused Ig concentrates as well as the immunomodulatory effect of Ig in patients with autoimmune and inflammatory diseases.
引用
收藏
页码:357 / 361
页数:5
相关论文
共 22 条
[1]   Intravenous immune globulin affects cytokine production in T lymphocytes and monocytes macrophages [J].
Andersson, J ;
SkansenSaphir, U ;
Sparrelid, E ;
Andersson, U .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1996, 104 :10-20
[2]  
AUKRUST P, 1994, BLOOD, V84, P2136
[3]   INTRAVENOUS USE OF GAMMA-GLOBULIN IN THE TREATMENT OF CHRONIC IMMUNE THROMBOCYTOPENIC PURPURA AS A MEANS TO DEFER SPLENECTOMY [J].
BUSSEL, JB ;
SCHULMAN, I ;
HILGARTNER, MW ;
BARANDUN, S .
JOURNAL OF PEDIATRICS, 1983, 103 (04) :651-654
[4]   INTRAVENOUS IMMUNOGLOBULIN MODULATES HUMAN MONONUCLEAR PHAGOCYTE TUMOR-NECROSIS-FACTOR-ALPHA PRODUCTION IN-VITRO [J].
DARVILLE, T ;
TABOR, D ;
SIMPSON, K ;
JACOBS, RF .
PEDIATRIC RESEARCH, 1994, 35 (04) :397-403
[5]   SELECTIVE INDUCTION OF INTERLEUKIN-1 RECEPTOR ANTAGONIST AND INTERLEUKIN-8 IN HUMAN MONOCYTES BY NORMAL POLYSPECIFIC IGG (INTRAVENOUS IMMUNOGLOBULIN) [J].
DESOUZA, VR ;
CARRENO, MP ;
KAVERI, SV ;
LEDUR, A ;
SADEGHI, H ;
CAVAILLON, JM ;
KAZATCHKINE, MD ;
HAEFFNERCAVAILLON, N .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1995, 25 (05) :1267-1273
[6]  
DWYER JM, 1992, NEW ENGL J MED, V326, P107
[7]   LIPOPOLYSACCHARIDE PRIMING OF HUMAN-NEUTROPHILS FOR AN ENHANCED RESPIRATORY BURST - ROLE OF INTRACELLULAR FREE CALCIUM [J].
FOREHAND, JR ;
PABST, MJ ;
PHILLIPS, WA ;
JOHNSTON, RB .
JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (01) :74-83
[8]   Effect of immunoglobulin therapy on phagocytosis by polymorphonuclear leucocytes in whole blood of neonates [J].
Fujiwara, T ;
Taniuchi, S ;
Hattori, K ;
Kobayashi, T ;
Kinoshita, Y ;
Kobayashi, Y .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1997, 107 (03) :435-439
[9]  
GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440
[10]  
HUIZINGA TWJ, 1990, BLOOD, V75, P1211