Single ricin detection by atomic force microscopy chemomechanical mapping

被引：16

作者：

Chen, Guojun ^{[1
,2
]}

Zhou, Jianfeng ^{[1
,2
]}

Park, Bosoon ^{[3
]}

Xu, Bingqian ^{[1
,2
]}

机构：

[1] Univ Georgia, Fac Engn, Single Mol Study Lab, Athens, GA 30602 USA

[2] Univ Georgia, Nanoscale Sci & Engn Ctr, Athens, GA 30602 USA

[3] USDA ARS, Russell Res Ctr, Athens, GA 30605 USA

来源：

APPLIED PHYSICS LETTERS | 2009年 / 95卷 / 04期

基金：

美国国家科学基金会;

关键词：

atomic force microscopy; bonds (chemical); dissociation; molecular biophysics; molecular configurations; proteins; RIBOSOME-INACTIVATING PROTEINS; MOLECULAR RECOGNITION; SPECTROSCOPY; AFM; LOCALIZATION; IMMUNOASSAY; CHEMISTRY; BIOLOGY; EVENTS;

D O I：

10.1063/1.3190197

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The authors report on a study of detecting ricin molecules immobilized on chemically modified Au (111) surface by chemomechanically mapping the molecular interactions with a chemically modified atomic force microscopy (AFM) tip. AFM images resolved the different fold-up conformations of single ricin molecule as well as their intramolecule structure of A- and B-chains. AFM force spectroscopy study of the interaction indicates that the unbinding force has a linear relation with the logarithmic force loading rate, which agrees well with calculations using one-barrier bond dissociation model.

引用

页数：3