Recombinant rabies virus as potential live-viral Vaccines for HIV-1

被引：98

作者：

Schnell, MJ

Foley, HD

Siler, CA

McGettigan, JP

Dietzschold, B

Pomerantz, RJ

机构：

[1] Thomas Jefferson Univ, Jefferson Med Coll, Ctr Human Virol, Dorrance H Hamilton Labs, Philadelphia, PA 19107 USA

[2] Thomas Jefferson Univ, Jefferson Med Coll, Dept Mol Pharmacol & Biochem, Dorrance H Hamilton Labs, Philadelphia, PA 19107 USA

[3] Thomas Jefferson Univ, Jefferson Med Coll, Dept Microbiol & Immunol, Dorrance H Hamilton Labs, Philadelphia, PA 19107 USA

[4] Thomas Jefferson Univ, Jefferson Med Coll, Dept Med, Dorrance H Hamilton Labs, Philadelphia, PA 19107 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 07期

关键词：

rhabdoviruses; RNA; envelope; gp160;

D O I：

10.1073/pnas.050589197

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recombinant, replication-competent rabies virus (RV) vaccine strain-based vectors were developed expressing HIV type I (HIV-1) envelope glycoprotein (gp160) from both a laboratory-adapted (CXCR4-tropic) and a primary (dual-tropic) HIV-1 isolate. An additional transcription stop/start unit within the RV genome was used to express HIV-1 gp160 in addition to the other RV proteins, The HIV-1 gp160 protein was stably and functionally expressed, as indicated by fusion of human T cell lines after infection with the recombinant RVs, Inoculation of mice with the recombinant RVs expressing HIV-1 gp160 induced a strong humoral response directed against the HIV-1 envelope protein after a single boost with recombinant HIV-1 gp120 protein. Moreover, high neutralization titers up to 1:800 against HIV-1 could be detected in the mouse sera. These data indicate that a live recombinant RV, a rhabdovirus, expressing HIV-1 gp160 may serve as an effective vector for an HIV-1 vaccine.

引用

页码：3544 / 3549

页数：6

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