Characterization of CYP71Z18 indicates a role in maize zealexin biosynthesis

被引：45

作者：

Mao, Hongjie ^{[1
]}

Liu, Jiang ^{[1
]}

Ren, Fei ^{[1
]}

Peters, Reuben J. ^{[2
]}

Wang, Qiang ^{[1
,3
]}

机构：

[1] Sichuan Agr Univ, Coll Agron, Chengdu 611130, Peoples R China

[2] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Roy J Carver Dept, Ames, IA 50011 USA

[3] Sichuan Agr Univ, Inst Ecol Agr, Chengdu 611130, Peoples R China

来源：

PHYTOCHEMISTRY | 2016年 / 121卷

关键词：

Maize; Zea mays; Poaceae; Biosynthesis; Terpenoid; Phytoalexin; P450; Zealexin; GENE-CLUSTER; ARABIDOPSIS-THALIANA; FUNCTIONAL-CHARACTERIZATION; DITERPENOID METABOLISM; TERPENOID PHYTOALEXINS; DIPHOSPHATE SYNTHASE; ESCHERICHIA-COLI; RICE; EXPRESSION; CYTOCHROME-P450;

D O I：

10.1016/j.phytochem.2015.10.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Maize (Zea mays) produces zealexins as phytoalexins, with the inducible production of these antibiotics providing biochemical protection against fungal infection. However, the biosynthesis of these sesquiter-penoids has remained unclear. In particular, it is unclear how the olefinic precursor, (S)-beta-macrocarpene produced by the characterized maize sesquiterpene synthases TPS6/11, is further elaborated to form the bioactive zealexins. The first step is likely to be conversion of carbon-15 (C15) from a methyl group to a carboxylic acid by a cytochrome P450 mono-oxygenase (CYP). In this study, CYP71Z18, whose transcription is strongly induced by fungal infection, was found to catalyze oxidation of C15 in (S)-beta-macrocarpene, forming zealexin A1. The inducible transcription of CYP71Z18 matches that observed for TPS6/11 and the accumulation of zealexins, which is consistent with a role for CYP71Z18 in sesquiterpenoid phytoalexin production. This completes identification of zealexin A1 biosynthesis, and represents the initial CYP identified for the production of maize terpenoid phytoalexins. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：4 / 10

页数：7

共 37 条

[1] Investigation of terpene diversification across multiple sequenced plant genomes [J].

Boutanaev, Alexander M. ;

Moses, Tessa ;

Zi, Jiachen ;

Nelson, David R. ;

Mugford, Sam T. ;

Peters, Reuben J. ;

Osbourn, Anne .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (01) :E81-E88

[2] CHEMICAL ACTIVATION OF HOST DEFENCE MECHANISMS AS A BASIS FOR CROP PROTECTION [J].

CARTWRIGHT, D ;

LANGCAKE, P ;

PRYCE, RJ ;

LEWORTHY, DP ;

RIDE, JP .

NATURE, 1977, 267 (5611) :511-513

[3] A modular approach for facile biosynthesis of labdane-related diterpenes [J].

Cyr, Anthony ;

Wilderman, P. Ross ;

Determan, Mara ;

Peters, Reuben J. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (21) :6684-+

[4] Reprogramming a maize plant: transcriptional and metabolic changes induced by the fungal biotroph Ustilago maydis [J].

Doehlemann, Gunther ;

Wahl, Ramon ;

Horst, Robin J. ;

Voll, Lars M. ;

Usadel, Bjoern ;

Poree, Fabien ;

Stitt, Mark ;

Pons-Kuehnemann, Joern ;

Sonnewald, Uwe ;

Kahmann, Regine ;

Kaemper, Joerg .

PLANT JOURNAL, 2008, 56 (02) :181-195

[5]

FAOSTAT, 2014, SUMM CROP PROD

[6] CLONING AND NUCLEOTIDE-SEQUENCE OF THE ISPA GENE RESPONSIBLE FOR FARNESYL DIPHOSPHATE SYNTHASE ACTIVITY IN ESCHERICHIA-COLI [J].

FUJISAKI, S ;

HARA, H ;

NISHIMURA, Y ;

HORIUCHI, K ;

NISHINO, T .

JOURNAL OF BIOCHEMISTRY, 1990, 108 (06) :995-1000

[7] The maize An2 gene is induced by Fusarium attack and encodes an ent-copalyl diphosphate synthase [J].

Harris, LJ ;

Saparno, A ;

Johnston, A ;

Prisic, S ;

Xu, M ;

Allard, S ;

Kathiresan, A ;

Ouellet, T ;

Peters, RJ .

PLANT MOLECULAR BIOLOGY, 2005, 59 (06) :881-894

[8] Novel Acidic Sesquiterpenoids Constitute a Dominant Class of Pathogen-Induced Phytoalexins in Maize [J].

Huffaker, Alisa ;

Kaplan, Fatma ;

Vaughan, Martha M. ;

Dafoe, Nicole J. ;

Ni, Xinzhi ;

Rocca, James R. ;

Alborn, Hans T. ;

Teal, Peter E. A. ;

Schmelz, Eric A. .

PLANT PHYSIOLOGY, 2011, 156 (04) :2082-2097

[9] Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis [J].

Kitaoka, Naoki ;

Wu, Yisheng ;

Xu, Meimei ;

Peters, Reuben J. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 99 (18) :7549-7558

[10] The Application of Synthetic Biology to Elucidation of Plant Mono-, Sesqui-, and Diterpenoid Metabolism [J].

Kitaoka, Naoki ;

Lu, Xuan ;

Yang, Bing ;

Peters, Reuben J. .

MOLECULAR PLANT, 2015, 8 (01) :6-16

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