'Shocking' developments in chick embryology:: electroporation and in ovo gene expression

被引：238

作者：

Itasaki, N ^{[1
]}

Bel-Vialar, S ^{[1
]}

Krumlauf, R ^{[1
]}

机构：

[1] Natl Inst Med Res, Dev Neurobiol Lab, London NW7 1AA, England

来源：

NATURE CELL BIOLOGY | 1999年 / 1卷 / 08期

关键词：

D O I：

10.1038/70231

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Efficient gene transfer by electroporation of chick embryos in ovo has allowed the development of new approaches to the analysis of gene regulation, function and expression, creating an exciting opportunity to build upon the classical manipulative advantages of the chick embryonic system. This method is applicable to other vertebrate embryos and is an important tool with which to address cell and developmental biology questions. Here we describe the technical aspects of in ovo electroporation, its different applications and future perspectives.

引用

页码：E203 / E207

页数：5

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