NG2 proteoglycan promotes endothelial cell motility and angiogenesis via engagement of galectin-3 and α3β1 integrin

被引：272

作者：

Fukushi, J ^{[1
]}

Makagiansar, IT ^{[1
]}

Stallcup, WB ^{[1
]}

机构：

[1] Burnham Inst, La Jolla, CA 92037 USA

来源：

MOLECULAR BIOLOGY OF THE CELL | 2004年 / 15卷 / 08期

关键词：

D O I：

10.1091/mbc.E04-03-0236

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The NG2 proteoglycan is expressed by microvascular pericytes in newly formed blood vessels. We have used in vitro and in vivo models to investigate the role of NG2 in cross-talk between pericytes and endothelial cells (EC). Binding of soluble NG2 to the EC surface induces cell motility and multicellular network formation in vitro and stimulates corneal angiogenesis in vivo. Biochemical data demonstrate the involvement of both galectin-3 and alpha3beta1 integrin in the EC response to NG2 and show that NG2, galectin-3, and alpha3beta1 form a complex on the cell surface. Transmembrane signaling via alpha3beta1 is responsible for EC motility and morphogenesis in this system. Galectin-3- dependent oligomrization may potentiate NG2-mediated activation of alpha3beta1. In conjunction with recent studies demonstrating the early involvement of pericytes in angiogenesis, these data suggest that pericyte-derived NG2 is an important factor in promoting EC migration and morphogenesis during the early stages of neovascularization.

引用

页码：3580 / 3590

页数：11

共 47 条

[1] The generation and characterization of a cell line derived from a sporadic renal angiomyolipoma - Use of telomerase to obtain stable populations of cells from benign neoplasms [J].

Arbiser, JL ;

Yeung, R ;

Weiss, SW ;

Arbiser, ZK ;

Amin, MB ;

Cohen, C ;

Frank, D ;

Mahajan, S ;

Herron, GS ;

Yang, JW ;

Onda, H ;

Zhang, HB ;

Bai, XH ;

Uhlmann, E ;

Loehr, A ;

Northrup, H ;

Au, P ;

Davis, I ;

Fisher, DE ;

Gutmann, DH .

AMERICAN JOURNAL OF PATHOLOGY, 2001, 159 (02) :483-491

[2]

Barritt DS, 2000, J CELL BIOCHEM, V79, P213, DOI 10.1002/1097-4644(20001101)79:2<213::AID-JCB50>3.0.CO

[3]

2-G

[4] Basic fibroblast growth factor-induced angiogenic phenotype in mouse endothelium - A study of aortic and microvascular endothelial cell lines [J].

Bastaki, M ;

Nelli, EE ;

DellEra, P ;

Rusnati, M ;

MolinariTosatti, MP ;

Parolini, S ;

Auerbach, R ;

Ruco, LP ;

Possati, L ;

Presta, M .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1997, 17 (03) :454-464

[5] Vascular development: Cellular and molecular regulation [J].

Beck, L ;

DAmore, PA .

FASEB JOURNAL, 1997, 11 (05) :365-373

[6]

Benjamin LE, 1998, DEVELOPMENT, V125, P1591

[7] Binding of the NG2 proteoglycan to type VI collagen and other extracellular matrix molecules [J].

Burg, MA ;

Tillet, E ;

Timpl, R ;

Stallcup, WB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (42) :26110-26116

[8]

Burg MA, 1998, J CELL PHYSIOL, V177, P299, DOI 10.1002/(SICI)1097-4652(199811)177:2<299::AID-JCP12>3.0.CO

[9]

2-5

[10] Cell contact-dependent activation of α3β1 integrin modulates endothelial cell responses to thrombospondin-1 [J].

Chandrasekaran, L ;

He, CZ ;

Al-Barazi, H ;

Krutzsch, HC ;

Iruela-Arispe, ML ;

Roberts, DD .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (09) :2885-2900

← 1 2 3 4 5 →