Alternative splicing within the TGF-β type I receptor gene (ALK-5) generates two major functional isoforms in vascular smooth muscle cells

被引：15

作者：

Agrotis, A ^{[1
]}

Condron, M ^{[1
]}

Bobik, A ^{[1
]}

机构：

[1] Alfred Hosp, Baker Med Res Inst, Cell Biol Lab, Prahran, Vic 8008, Australia

来源：

FEBS LETTERS | 2000年 / 467卷 / 01期

基金：

英国医学研究理事会;

关键词：

type I transforming growth factor-beta receptor; (ALK-5); splice variant; vascular smooth muscle;

D O I：

10.1016/S0014-5793(00)01132-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We have identified in rat vascular smooth muscle cells (sn rcs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length proteins differ from each other only by the in-frame presence or absence of Gly-Pro-Phe-Ser residues adjacent to their transmembrane domain. Stable expression of these alternate ALK-5 isoforms in ALK-5-deficient cells demonstrated that both were competent in signaling TGF-beta-induced growth inhibition and gene transcription, but with an apparently distinct potency. Our data suggest that alternate splicing within the ALK-5 gene is an important mechanism whereby SMCs may regulate their response to TGF-beta. (C) 2000 Federation of European Biochemical Societies.

引用

页码：128 / 132

页数：5

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