Solution NMR of membrane proteins:: practice and challenges

被引：187

作者：

Sanders, Charles R. ^{[1
]}

Soennichsen, Frank

机构：

[1] Vanderbilt Univ, Dept Biochem, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Ctr Struct Biol, Nashville, TN 37232 USA

[3] Case Western Reserve Univ, Dept Physiol & Biophys, Cleveland, OH 44106 USA

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 2006年 / 44卷 / S24-S40期

关键词：

NMR; membrane; proteins; solution; review; detergent; detergents; micelles; amphipols; lipopeptides; bicelles; TROSY; labeling; deuteration; structure; backbone; fold;

D O I：

10.1002/mrc.1816

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This review focuses upon the application of solution NMR methods to multispan integral membrane proteins, particularly with respect to determination of global folds by this approach. Practical methods are described along with the special difficulties that confront the application of solution NMR to proteins that dwell in the netherworld of the lipid bilayer. Copyright (C) 2006 John Wiley & Sons, Ltd.

引用

页码：S24 / S40

页数：17

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