Analysis of particle bombardment parameters to optimise DNA delivery into wheat tissues

被引：102

作者：

Rasco-Gaunt, S ^{[1
]}

Riley, A ^{[1
]}

Barcelo, P ^{[1
]}

Lazzeri, PA ^{[1
]}

机构：

[1] IACR Rothasted, Dept Biochem & Physiol, Harpenden AL5 2JQ, Herts, England

来源：

PLANT CELL REPORTS | 1999年 / 19卷 / 02期

关键词：

Triticum aestivum; DNA delivery; particle bombardment; transient GUS expression;

D O I：

10.1007/s002990050721

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to wheat (Triticum aestivum L.) scutellum and inflorescence tissue. The main factors studied were the DNA/gold precipitation process, bombardment parameters and tissue culture variables. Efficiency of DNA (uidA gene) delivery was assessed by scoring transient GUS expression in bombarded tissues. Of the parameters analysed, amount of plasmid DNA, spermidine concentration, presence of Catt ions, calcium chloride concentration, amount of gold particles, gold particle size, acceleration pressure, chamber vacuum pressure, bombardment distance, osmotic conditioning of tissues and type of auxin had a clear influence on transient gene expression. A bombardment procedure suitable for elite wheat varieties was developed which allowed high-efficiency DNA delivery combined with reduced damage to target tissues.

引用

页码：118 / 127

页数：10

共 23 条

[1]

Altpeter F, 1996, PLANT CELL REP, V16, P12, DOI 10.1007/BF01275440

[2]

Barcelo Pilar, 1995, V49, P113

[3] Transformation of wheat with high molecular weight subunit genes results in improved functional properties [J].

Barro, F ;

Rooke, L ;

Békés, F ;

Gras, P ;

Tatham, AS ;

Fido, R ;

Lazzeri, PA ;

Shewry, PR ;

Barceló, P .

NATURE BIOTECHNOLOGY, 1997, 15 (12) :1295-1299

[4] FERTILE TRANSGENIC WHEAT FROM MICROPROJECTILE BOMBARDMENT OF SCUTELLAR TISSUE [J].

BECKER, D ;

BRETTSCHNEIDER, R ;

LORZ, H .

PLANT JOURNAL, 1994, 5 (02) :299-307

[5] Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants [J].

Christensen, AH ;

Quail, PH .

TRANSGENIC RESEARCH, 1996, 5 (03) :213-218

[6]

HUNOLD R, 1994, PLANT J, V5, P593, DOI 10.1046/j.1365-313X.1994.5040593.x

[7]

IGLESIAS VA, 1994, PLANTA, V192, P84

[8]

Jain RK, 1996, PLANT CELL REP, V15, P963, DOI 10.1007/BF00231597

[9] FACTORS INFLUENCING GENE DELIVERY INTO ZEA-MAYS CELLS BY HIGH-VELOCITY MICROPROJECTILES [J].

KLEIN, TM ;

GRADZIEL, T ;

FROMM, ME ;

SANFORD, JC .

BIO-TECHNOLOGY, 1988, 6 (05) :559-563

[10]

LAZZERI PA, 1993, BIOTECHNOL GENET ENG, V2, P79

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