Differential requirements for actin during yeast and mammalian endocytosis

被引：167

作者：

Aghamohammadzadeh, Soheil ^{[1
]}

Ayscough, Kathryn R. ^{[1
]}

机构：

[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Western Bank, Sheffield S10 2TN, S Yorkshire, England

来源：

NATURE CELL BIOLOGY | 2009年 / 11卷 / 08期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

PPZ PROTEIN PHOSPHATASES; CELL POLARITY; CYTOSKELETON; INVAGINATION; RECRUITMENT; PATHWAY;

D O I：

10.1038/ncb1918

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Key features of clathrin-mediated endocytosis have been conserved across evolution. However, endocytosis in Saccharomyces cerevisiae is completely dependent on a functional actin cytoskeleton, whereas actin appears to be less critical in mammalian cell endocytosis. We reveal that the fundamental requirement for actin in the early stages of yeast endocytosis is to provide a strong framework to support the force generation needed to direct the invaginating plasma membrane into the cell against turgor pressure. By providing osmotic support, pressure differences across the plasma membrane were removed and this reduced the requirement for actin-bundling proteins in normal endocytosis. Conversely, increased turgor pressure in specific yeast mutants correlated with a decreased rate of endocytic patch invagination.

引用

页码：1039 / U283

页数：5

共 14 条

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