The coronatine-insensitive 1 mutation reveals the hormonal signaling interaction between abscisic acid and methyl jasmonate in arabidopsis guard cells.: Specific impairment of ion channel activation and second messenger production

被引:265
作者
Munemasa, Shintaro
Oda, Kenji
Watanabe-Sugimoto, Megumi
Nakamura, Yoshimasa
Shimoishi, Yasuaki
Murata, Yoshiyuki [1 ]
机构
[1] Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan
[2] Res Inst Biol Sci OKAYAMA, Okayama 7161241, Japan
[3] Okayama Univ, Dept Agr, Okayama 7008530, Japan
关键词
D O I
10.1104/pp.106.091298
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Methyl jasmonate (MeJA) elicits stomatal closing similar to abscisic acid (ABA), but whether the two compounds use similar or different signaling mechanisms in guard cells remains to be clarified. We investigated the effects of MeJA and ABA on second messenger production and ion channel activation in guard cells of wild-type Arabidopsis ( Arabidopsis thaliana) and MeJA-insensitive coronatine-insensitive 1 (coi1) mutants. The coi1 mutation impaired MeJA-induced stomatal closing but not ABA-induced stomatal closing. MeJA as well as ABA induced production of reactive oxygen species (ROS) and nitric oxide (NO) in wild-type guard cells, whereas MeJA did not induce production of ROS and NO in coi1 guard cells. The experiments using an inhibitor and scavengers demonstrated that both ROS and NO are involved in MeJA-induced stomatal closing as well as ABA-induced stomatal closing. Not only ABA but also MeJA activated slow anion channels and Ca2+ permeable cation channels in the plasma membrane of wild-type guard cell protoplasts. However, in coi1 guard cell protoplasts, MeJA did not elicit either slow anion currents or Ca2+ permeable cation currents, but ABA activated both types of ion channels. Furthermore, to elucidate signaling interaction between ABA and MeJA in guard cells, we examined MeJA signaling in ABA-insensitive mutant ABA-insensitive 2 (abi2-1), whose ABA signal transduction cascade has some disruption downstream of ROS production and NO production. MeJA also did not induce stomatal closing but stimulated production of ROS and NO in abi2-1. These results suggest that MeJA triggers stomatal closing via a receptor distinct from the ABA receptor and that the coi1 mutation disrupts MeJA signaling upstream of the blanch point of ABA signaling and MeJA signaling in Arabidopsis guard cells.
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页码:1398 / 1407
页数:10
相关论文
共 50 条
[1]   Arabidopsis abi1-1 and abi2-1 phosphatase mutations reduce abscisic acid-induced cytoplasmic calcium rises in guard cells [J].
Allen, GJ ;
Kuchitsu, K ;
Chu, SP ;
Murata, Y ;
Schroeder, JI .
PLANT CELL, 1999, 11 (09) :1785-1798
[2]   Cameleon calcium indicator reports cytoplasmic calcium dynamics in Arabidopsis guard cells [J].
Allen, GJ ;
Kwak, JM ;
Chu, SP ;
Llopis, J ;
Tsien, RY ;
Harper, JF ;
Schroeder, JI .
PLANT JOURNAL, 1999, 19 (06) :735-747
[3]   Hypersensitivity of abscisic acid-induced cytosolic calcium increases in the Arabidopsis farnesyltransferase mutant era1-2 [J].
Allen, GJ ;
Murata, Y ;
Chu, SP ;
Nafisi, M ;
Schroeder, JI .
PLANT CELL, 2002, 14 (07) :1649-1662
[4]   Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant [J].
Allen, GJ ;
Chu, SP ;
Schumacher, K ;
Shimazaki, CT ;
Vafeados, D ;
Kemper, A ;
Hawke, SD ;
Tallman, G ;
Tsien, RY ;
Harper, JF ;
Chory, J ;
Schroeder, JI .
SCIENCE, 2000, 289 (5488) :2338-2342
[5]   COI1-DEPENDENT EXPRESSION OF AN ARABIDOPSIS VEGETATIVE STORAGE PROTEIN IN FLOWERS AND SILIQUES AND IN RESPONSE TO CORONATINE OR METHYL JASMONATE [J].
BENEDETTI, CE ;
XIE, DX ;
TURNER, JG .
PLANT PHYSIOLOGY, 1995, 109 (02) :567-572
[6]  
BLATT MR, 1993, PLANTA, V191, P330, DOI 10.1007/BF00195690
[7]   ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis [J].
Bright, J ;
Desikan, R ;
Hancock, JT ;
Weir, IS ;
Neill, SJ .
PLANT JOURNAL, 2006, 45 (01) :113-122
[8]   JASMONIC ACID DISTRIBUTION AND ACTION IN PLANTS - REGULATION DURING DEVELOPMENT AND RESPONSE TO BIOTIC AND ABIOTIC STRESS [J].
CREELMAN, RA ;
MULLET, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) :4114-4119
[9]   A new role for an old enzyme:: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana [J].
Desikan, R ;
Griffiths, R ;
Hancock, J ;
Neill, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (25) :16314-16318
[10]   The Arabidopsis mutant cev1 has constitutively active jasmonate and ethylene signal pathways and enhanced resistance to pathogens [J].
Ellis, C ;
Turner, JG .
PLANT CELL, 2001, 13 (05) :1025-1033