Short TE MR microscopy: Accurate measurement and zonal differentiation of normal hyaline cartilage

The purpose of this study was to use MR imaging to accurately measure the thickness of hyaline cartilage and determine the MR contrast parameters for differentiation of cartilage zones in normal human cartilage samples, Cartilage samples were examined using three dimensional spin-echo MR microscopy at 9.4 T with a voxel size of 31 x 31 x 300 pm, Effects of T-2 signal loss, susceptibility, and partial volume on measured thickness of cartilage were investigated. Thickness measurements were obtained on corresponding histological sections for comparison. Optimal contrast parameters for delineation of cartilage zones were evaluated using magnetization transfer, inversion recovery, T-2, and T-2 contrast, T-2 relaxation losses were identified as the primary source of discrepancy between the measured thickness of cortical bone and hyaline cartilage, Good contrast for zonal differentiation was obtained using T-1 weighting. We conclude that images obtained using short TE MR microscopy can be used to accurately measure cartilage and bone thickness in human specimens, and can demonstrate zones within normal cartilage.