Adhesion and morphology of fibroblastic cells cultured on different polymeric biomaterials

被引:29
作者
Lombello, CB
Santos, AR
Malmonge, SM
Barbanti, SH
Wada, MLF
Duek, EAR
机构
[1] Inst Biol, Dept Cell Biol, Campinas, SP, Brazil
[2] DDPP Ctr Technol, Labiomec, Campinas, SP, Brazil
[3] UNICAMP, Fac Mech Engn, Dept Mat Engn, Campinas, SP, Brazil
[4] Pontificial Catholic Univ Sao Paulo, Coll Biol Sci, Dept Physiol Sci, Sorocaba, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
D O I
10.1023/A:1016552413295
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Cell adhesion is influenced by the physical and chemical characteristics of the materials used as substrate for cell culturing. In this work, we evaluated the influence of the morphological and chemical characteristics of different polymeric substrates on the adhesion and morphology of fibroblastic cells. Cell growth on poly (L-lactic acid) [PLLA] membranes and poly(2-hydroxy ethyl methacrylate) [polyHEMA], poly(2-hydroxy ethyl methacrylate)-cellulose acetate [polyHEMA-CA] and poly(2-hydroxy ethyl methacrylate)-poly(methyl methacrylate-co-acrylic acid) [polyHEMA-poly(MMA-co-AA)] hydrogels of different densities and pore diameters was examined. Cells adhered preferentially to more negativaly charged substrates, with polyHEMA hydrogels being more adhesive than the other substractes. The pores present in PLLA membranes did not interfere with adhesion, but the cells showed a distinctive morphology on each membrane. (C) 2002 Kluwer Academic Publishers.
引用
收藏
页码:867 / 874
页数:8
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