Detection of organophosphates using an acetyl cholinesterase (AChE) coated microcantilever

被引：15

作者：

Yan, XD

Tang, YJ

Ji, HF ^{[1
]}

Lvov, Y

Thundat, T

机构：

[1] Louisiana Tech Univ, Inst Micromfg, Ruston, LA 71272 USA

[2] Oak Ridge Natl Lab, Div Life Sci, Oak Ridge, TN USA

来源：

INSTRUMENTATION SCIENCE & TECHNOLOGY | 2004年 / 32卷 / 02期

关键词：

microcantilever; acetyl cholinesterase (AChE); organophosphate detection; adsorption-induced stress; self-assembled monolayers (SAMs);

D O I：

10.1081/CI-120028770

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A microcantilever modified with a layer of acetyl cholinesterase (AChE) responding to paraoxon is reported in this work. Acetyl cholinesterase was immobilized on a microcantilever through a cross linker to a monolayer on gold surface of the microcantilever. The microcantilever underwent a maximum of 7 nm bending due to the inhibition of AChE by paraoxon that slightly changed the conformation of AChE.

引用

收藏

页码：175 / 183

页数：9

相关论文

共 15 条

[1] A biosensor based on micromechanical interrogation of living cells [J].

Antonik, MD ;

DCosta, NP ;

Hoh, JH .

IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 1997, 16 (02) :66-72

[2] A chemical sensor based on a microfabricated cantilever array with simultaneous resonance-frequency and bending readout [J].

Battiston, FM ;

Ramseyer, JP ;

Lang, HP ;

Baller, MK ;

Gerber, C ;

Gimzewski, JK ;

Meyer, E ;

Güntherodt, HJ .

SENSORS AND ACTUATORS B-CHEMICAL, 2001, 77 (1-2) :122-131

[3] Acetylcholinesterase engineering for detection of insecticide residues [J].

Boublik, Y ;

Saint-Aguet, P ;

Lougarre, A ;

Arnaud, M ;

Villatte, F ;

Estrada-Mondaca, S ;

Fournier, D .

PROTEIN ENGINEERING, 2002, 15 (01) :43-50

[4]

Brown MA, 1998, J APPL TOXICOL, V18, P393, DOI 10.1002/(SICI)1099-1263(199811/12)18:6<393::AID-JAT528>3.0.CO

[5]

2-0

[6] ADSORPTION-INDUCED SURFACE STRESS AND ITS EFFECTS ON RESONANCE FREQUENCY OF MICROCANTILEVERS [J].

CHEN, GY ;

THUNDAT, T ;

WACHTER, EA ;

WARMACK, RJ .

JOURNAL OF APPLIED PHYSICS, 1995, 77 (08) :3618-3622

[7] SELF-ASSEMBLED MONOLAYERS AND ENZYME ELECTRODES - PROGRESS, PROBLEMS AND PROSPECTS [J].

CREAGER, SE ;

OLSEN, KG .

ANALYTICA CHIMICA ACTA, 1995, 307 (2-3) :277-289

[8] Translating biomolecular recognition into nanomechanics [J].

Fritz, J ;

Baller, MK ;

Lang, HP ;

Rothuizen, H ;

Vettiger, P ;

Meyer, E ;

Güntherodt, HJ ;

Gerber, C ;

Gimzewski, JK .

SCIENCE, 2000, 288 (5464) :316-318

[9] Atomic force microscopy with patterned cantilevers and tip arrays: Force measurements with chemical arrays [J].

Green, JBD ;

Lee, GU .

LANGMUIR, 2000, 16 (08) :4009-4015

[10]

Jaffrezic-Renault N., 2001, SENSORS, V1, P60, DOI DOI 10.3390/S10100060