Chemokine CCL20 enhances the growth of HuH7 cells via phosphorylation of p44/42 MAPK in vitro

被引:52
作者
Fujii, H [1 ]
Itoh, Y [1 ]
Yamaguchi, K [1 ]
Yamauchi, N [1 ]
Harano, Y [1 ]
Nakajima, T [1 ]
Minami, M [1 ]
Okanoue, T [1 ]
机构
[1] Kyoto Prefectural Univ Med, Grad Sch Med Sci Mol Gastroenterol & Hepatol, Kyoto, Japan
基金
日本学术振兴会;
关键词
HuH7; CCR6; CCL20; p44/42; MAPK;
D O I
10.1016/j.bbrc.2004.07.207
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
CCR6 is the receptor of chemokine CCL20. In the present study, we demonstrated that the surface expression of CCR6 was enhanced on the human HCC cell lines (HuH7, PLC/PRF/5, and HepG2) especially on HuH7 cells, but not on HLE or HLF Cells. These HCC cell lines (HuH7, PLC/PRF/5, and HepG2) especially the HuH7 cells secreted a significant amount of CCL20 spontaneously, whereas HLE or HLF did not. Stimulation by CCL20 up-regulated the mRNA expression of CCR6 in HuH7 cells and significantly enhanced the growth of HuH7 cells. CCL20-stimulated growth of HuH7 cells was abrogated by the inhibition of downstream signal transduction pathway mediated by p44/42 MAPK, but not by p38 MAPK or SAPK/JNK. CCR6 expression in human HCC tissues was confirmed by RT-PCR. These results indicate that the growth of a proportion of human HCC cells may be mediated by CCL20-CCR6 axis, like HuH7 cells, in an autocrine or paracrine manner. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:1052 / 1058
页数:7
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