Reversed-phase liquid chromatography on a microchip with sample injector and monolithic silica column

被引：38

作者：

Ishida, Akihiko ^{[1
]}

Yoshikawa, Takahiro ^{[1
]}

Natsume, Masamichi ^{[1
]}

Kamidate, Tamio ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Engn, Div Biotechnol & Macromol Chem, Sapporo, Hokkaido 0608628, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1132卷 / 1-2期

基金：

日本学术振兴会;

关键词：

chip technology; microchip LC; monolithic silica; pressure-driven flow; turn geometry; catechins;

D O I：

10.1016/j.chroma.2006.07.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In micro total analysis systems, liquid chromatography (LC) works under pressure-driven flow is the essential analysis component. There were not, however, much works on microchip LC. Here we developed a microchip for reversed-phase LC using porous monolithic silica. The chip consisted of a double T-shaped injector and a similar to 40-cm serpentine separation channel. The octadecyl-modified monolithic silica was prepared in the specified part of the channel on the microchip using sol-gel process. Furthermore, the effect of geometry of turn sections on band dispersion at turns was examined under pressure-driven flow. High separation efficiencies of 15,000-18,000 plates/m for catechins were obtained using the LC chip. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：90 / 98

页数：9

共 51 条

[1]

AHUJA S, 1993, TRACE ULTRATRACE ANA, P25

[2] Micro total analysis systems. 2. Analytical standard operations and applications [J].

Auroux, PA ;

Iossifidis, D ;

Reyes, DR ;

Manz, A .

ANALYTICAL CHEMISTRY, 2002, 74 (12) :2637-2652

[3] Functional hydrogel structures for autonomous flow control inside microfluidic channels [J].

Beebe, DJ ;

Moore, JS ;

Bauer, JM ;

Yu, Q ;

Liu, RH ;

Devadoss, C ;

Jo, BH .

NATURE, 2000, 404 (6778) :588-+

[4]

BING H, 1998, ANAL CHEM, V70, P3790

[5] Microchip-based purification of DNA from biological samples [J].

Breadmore, MC ;

Wolfe, KA ;

Arcibal, IG ;

Leung, WK ;

Dickson, D ;

Giordano, BC ;

Power, ME ;

Ferrance, JP ;

Feldman, SH ;

Norris, PM ;

Landers, JP .

ANALYTICAL CHEMISTRY, 2003, 75 (08) :1880-1886

[6] A pressure driven injection system for an ultra-flat chromatographic microchannel [J].

Chmela, E ;

Blom, MT ;

Gardeniers, JGE ;

van den Berg, A ;

Tijssen, R .

LAB ON A CHIP, 2002, 2 (04) :235-241

[7] Microchip devices for high-efficiency separations [J].

Culbertson, CT ;

Jacobson, SC ;

Ramsey, JM .

ANALYTICAL CHEMISTRY, 2000, 72 (23) :5814-5819

[8] Dispersion sources for compact geometries on microchips [J].

Culbertson, CT ;

Jacobson, SC ;

Ramsey, JM .

ANALYTICAL CHEMISTRY, 1998, 70 (18) :3781-3789

[9] A low dispersion geometry for microchip separation devices [J].

Dutta, D ;

Leighton, DT .

ANALYTICAL CHEMISTRY, 2002, 74 (05) :1007-1016

[10] Electroosmosis- and pressure-driven chromatography in chips using continuous beds [J].

Ericson, C ;

Holm, J ;

Ericson, T ;

Hjertén, S .

ANALYTICAL CHEMISTRY, 2000, 72 (01) :81-87

← 1 2 3 4 5 6 →