Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase

被引：6

作者：

Chimnaronk, S

Jeppesen, MG

Shimada, N

Suzuki, T

Nyborg, J

Watanabe, K

机构：

[1] Aarhus Univ, Dept Mol Biol, DK-8000 Aarhus C, Denmark

[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Chiba 2778562, Japan

[3] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷

关键词：

D O I：

10.1107/S0907444904011217

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitochondrial seryl-tRNA synthetase (mt SerRS) from Bos taurus was overexpressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion method. Crystals grew in a very narrow range of conditions using PEG 8000 as precipitant at room temperature. An appropriate concentration of lithium sulfate was critical for crystal nucleation. Crystals diffracted well beyond a resolution of 1.6 Angstrom and were found to belong to the orthorhombic space group C222(1), with unit-cell parameters a = 79.89, b = 230.42, c = 135.60 Angstrom. There is one dimer (M-r similar or equal to 113 kDa) in the asymmetric unit, with a solvent content of 55%. Efforts to solve the phase problem by molecular replacement are under way.

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页码：1319 / 1322

页数：4

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