Bone morphogenic protein-2 gene therapy for mandibular distraction osteogenesis

被引：81

作者：

Ashinoff, RL ^{[1
]}

Cetrulo, CL ^{[1
]}

Galiano, RD ^{[1
]}

Dobryansky, M ^{[1
]}

Bhatt, KA ^{[1
]}

Ceradini, DJ ^{[1
]}

Michaels, J ^{[1
]}

McCarthy, JG ^{[1
]}

Gurtner, GC ^{[1
]}

机构：

[1] NYU, Med Ctr, Inst Reconstruct Plast Surg, Lab Microvasc Res & Vasc Tissue Engn, New York, NY 10016 USA

来源：

ANNALS OF PLASTIC SURGERY | 2004年 / 52卷 / 06期

关键词：

BMP-2; gene therapy; distraction osteogenesis;

D O I：

10.1097/01.sap.0000123023.28874.1e

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Distraction osteogenesis (DO) requires a long consolidation period and has a low but real failure rate. Bone morphogenic proteins (BMPs) accelerate bone deposition in fractures and critical-sized bone defects, but their effects on mandibular DO are unknown. We investigated the effect of local delivery of adenovirus containing the gene for BMP-2 (Adbmp-2) on mandibular DO in a rat model. Rats (n = 54) were distracted to 3 mm over 6 days. At the start of consolidation (POD 10), Adbmp-2 or adenovirus containing the lacZ gene (AdlacZ) was injected directly into the distraction zone. After 1, 2, and 4 weeks of consolidation, mandibles were evaluated for amount of bone deposition. Adbmp-2-treated specimens demonstrated an increased amount of new bone formation by radiographic, histologic, and histomorphometric analysis. This study demonstrates that local, adenovirally-mediated delivery of BMP-2 can increase bone deposition during DO, potentially shortening consolidation and enhancing DO in poorly healing mandibles, such as occurs postirradiation.

引用

页码：585 / 591

页数：7

共 23 条

[1] The use of bone morphogenetic protein gene therapy in craniofacial bone repair [J].