Uptake and accumulation of ammonium by Alexandrium catenella during nutrient pulses

被引:18
作者
Collos, Y.
Lespilette, M.
Vaquer, A.
Laabir, M.
Pastoureaud, A.
机构
[1] Univ Montpellier 2, CNRS, UMR 5119, Lab Ecosyst Lagunaires, F-34095 Montpellier 5, France
[2] IFREMER, Lab Environm Ressources, LR, F-34203 Sete, France
关键词
Alexandrium catenella; ammonium accumulation; ammonium uptake; carbon fixation; nitrogen budget; strain variability;
D O I
10.2989/18142320609504169
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Field observations in Thau Lagoon, southern France, indicate that the growth of natural populations of Alexandrium catenella during blooms is limited by nitrogen and exhibits a storage rather than a growth response to an ammonium pulse. Therefore, ammonium uptake and accumulation under transient conditions were investigated in detail in laboratory cultures. Following nitrogen exhaustion from the medium, ammonium pulses of varying magnitudes were induced, and measurements of extra- and intra-cellular ammonium were carried out for 24-72h along with measurements of ammonium incorporation (N-15 tracer) and inorganic carbon fixation (C-13 tracer). During vegetative growth, values of intra-cellular ammonium reached 30% (Strain TL01) and 2% (Strain ACT03) of cell nitrogen. When ammonium was available in the culture medium, ammonium uptake estimated by the N-15 tracer technique accounted for only 65% of the decrease in external ammonium. This discrepancy is probably due to organic nitrogen excretion. Once external ammonium was exhausted, the observed isotopic dilution of both C-13 and N-15 cell content indicated uptake of a compound containing both unlabelled carbon and nitrogen atoms.
引用
收藏
页码:313 / 318
页数:6
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