Development of RGA-CAPS markers and genetic mapping of candidate genes for sugarcane mosaic virus resistance in maize

被引：36

作者：

Quint, M ^{[1
]}

Mihaljevic, R ^{[1
]}

Dussle, CM ^{[1
]}

Xu, ML ^{[1
]}

Melchinger, AE ^{[1
]}

Lübberstedt, T ^{[1
]}

机构：

[1] Univ Hohenheim, Inst Plant Breeding Seed Sci & Populat Genet, D-70593 Stuttgart, Germany

来源：

THEORETICAL AND APPLIED GENETICS | 2002年 / 105卷 / 2-3期

关键词：

RGA; SNP; CAPS; SCMV; maize;

D O I：

10.1007/s00122-002-0953-x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Three previously published resistance gene analogues (RGAs), pic13, pic21 and pic19, were mapped in relation to sugarcane mosaic virus (SCMV) resistance genes (Scmv1, Scmv2) in maize. We cloned these RGAs from six inbreds including three SCMV-resistant lines (D21, D32, FAP1360A) and three SCMV-susceptible lines (D145, D408, F7). Pairwise sequence alignments among the six inbreds revealed a frequency of one single nucleotide polymorphism (SNP) per 33 bp for the three RGAs, indicating a high degree of polymorphism and a high probability of success in converting RGAs into co-dominant cleaved amplified polymorphic sequence (CAPS) markers compared to other sequences. SNPs were used to develop CAPS markers for mapping of the three RGAs in relation to Scmv1 (chromosome 6) and Scmv2 (chromosome 3), and for pedigree analyses of resistant inbred lines. By genetic mapping pic21 was shown to be different from Scmv2, whereas pic19 and pic13 are still candidates for Scmv1 and Scmv2, respectively, due to genetic mapping and consistent restriction patterns of ancestral lines.

引用

页码：355 / 363

页数：9

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