Evolution and assembly of an extremely scrambled gene

被引：48

作者：

Landweber, LF ^{[1
]}

Kuo, TC ^{[1
]}

Curtis, EA ^{[1
]}

机构：

[1] Princeton Univ, Dept Ecol & Evolut Biol, Princeton, NJ 08544 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 07期

关键词：

gene rearrangement; DNA polymerase alpha; hypotrich; ciliate; DNA computing;

D O I：

10.1073/pnas.040574697

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The process of gene unscrambling in hypotrichous ciliates represents one of nature's ingenious solutions to the problem of gene assembly. With some essential genes scrambled in as many as 51 pieces, these ciliates rely on sequence and structural cues to rebuild their fragmented genes and genomes. Here we report the complex pattern of scrambling in the DNA polymerase alpha gene of Stylonychia lemnae, The germline (micronuclear) copy of this gene is broken into 48 pieces with 47 dispersed over two loci, with no asymmetry in the placement of coding segments on either strand. Direct repeats present at the boundaries between coding and noncoding sequences provide pointers to help guide assembly of the functional (macronuclear) gene. We investigate the evolution of this complex gene in three hypotrichous species.

引用

页码：3298 / 3303

页数：6

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