Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap

被引:237
作者
Ho, Chen-Ta
Lin, Ruei-Zeng
Chang, Wen-Yu
Chang, Hwan-You
Liu, Cheng-Hsien [1 ]
机构
[1] Natl Tsing Hua Univ, Dept Power Mech Engn, Hsinchu 300, Taiwan
[2] Natl Tsing Hua Univ, Inst Mol Med, Hsinchu 300, Taiwan
[3] Natl Tsing Hua Univ, Microelectromech Syst Inst, Hsinchu 300, Taiwan
关键词
D O I
10.1039/b602036d
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Biomimetic heterogeneous patterning of hepatic and endothelial cells, which start from randomly distributed cells inside the microfluidic chamber, via the chip design of enhanced field-induced dielectrophoresis (DEP) trap is demonstrated and reported in this paper. The concentric-stellate-tip electrode array design in this chip generates radial-pattern electric fields for the DEP manipulation of the live liver cells. By constructing the geometric shape and the distribution of stellate tips, the DEP electrodes enhance the desired spatial electric-field gradients to guide and snare individual cells to form the desired biomimetic pattern. With this proposed microfluidic chip design, the original randomly distributed hepatocytes inside the microfluidic chamber can be manipulated in parallel and align into the desired pearl-chain array pattern. This radial pattern mimics the lobular morphology of real liver tissue. The endothelial cells, then, are snared into the additional pearl-chain array and settle at the space in-between the previous hepatic pearl-chain array. By this cell-lab chip, we demonstrate the in vitro reconstruction of the heterogeneous lobule-mimetic radial pattern with good cell viability after cell patterning. This work reports the rapid in-parallel patterning of the dual types of live liver cells via the enhanced DEP trap inside the microfluidic chip.
引用
收藏
页码:724 / 734
页数:11
相关论文
共 46 条
[1]   Photo- and electropatterning of hydrogel-encapsulated living cell arrays [J].
Albrecht, DR ;
Tsang, VL ;
Sah, RL ;
Bhatia, SN .
LAB ON A CHIP, 2005, 5 (01) :111-118
[2]   Engineering liver therapies for the future [J].
Allen, JW ;
Bhatia, SN .
TISSUE ENGINEERING, 2002, 8 (05) :725-737
[3]   Building structured biomaterials using AC electrokinetics [J].
Alp, B ;
Andrews, JS ;
Mason, VP ;
Thompson, IP ;
Wolowacz, R ;
Markx, GH .
IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 2003, 22 (06) :91-97
[4]   Microfabrication and microfluidics for tissue engineering: state of the art and future opportunities [J].
Andersson, H ;
van den Berg, A .
LAB ON A CHIP, 2004, 4 (02) :98-103
[5]  
Atala A., 2001, Methods of tissue engineering, V1st
[6]   Engineering cellular microenvironments to cell-based drug testing improve [J].
Bhadriraju, K ;
Chen, CS .
DRUG DISCOVERY TODAY, 2002, 7 (11) :612-620
[7]  
Bhatia SN, 1997, J BIOMED MATER RES, V34, P189, DOI 10.1002/(SICI)1097-4636(199702)34:2<189::AID-JBM8>3.0.CO
[8]  
2-M
[9]   Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems [J].
Chiu, DT ;
Jeon, NL ;
Huang, S ;
Kane, RS ;
Wargo, CJ ;
Choi, IS ;
Ingber, DE ;
Whitesides, GM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) :2408-2413
[10]   Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array [J].
Choi, S ;
Park, JK .
LAB ON A CHIP, 2005, 5 (10) :1161-1167