Ultrasound-induced cell membrane porosity

被引:283
作者
Deng, CX [1 ]
Sieling, F
Pan, H
Cui, JM
机构
[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Cardiac Bioelect Res & Training Ctr, Cleveland, OH 44106 USA
关键词
sonoporation; cell membrane; ultrasound contrast agent; drug delivery; gene delivery;
D O I
10.1016/j.ultrasmedbio.2004.01.005
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
Recent studies of ultrasound (US) methods for targeted drug delivery and nonviral gene transfection revealed new, advantageous possibilities. These studies utilized US contrast agents, commonly stabilized micro-bubbles, to facilitate delivery and suggested that US delivery resulted from cell sonoporation, the formation of temporary pores in the cell membrane induced by US. Using voltage clamp techniques, we obtained real-time measurements of sonoporation of single Xenopus oocyte in the presence of Optison(TM), an agent consisting of albumin-shelled C,F, gas bubbles (mean diameter 3.2 mum). Ultrasound increased the transmembrane current as a direct result of decreased membrane resistance due to pore formation. We observed a distinct delay of sonoporation following US activation and characteristic stepwise increases of transmembrane current throughout US duration. We discovered that the resealing of cell membrane following US exposure required Ca2+ entering the cell through US-induced pores. (E-mail: cxd54@cwru.edu) (C) 2004 World Federation for Ultrasound in Medicine Biology.
引用
收藏
页码:519 / 526
页数:8
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