Differential regulation of CHIF mRNA by potassium intake and aldosterone

被引:33
作者
Wald, H [1 ]
Popovtzer, MM [1 ]
Garty, H [1 ]
机构
[1] WEIZMANN INST SCI, DEPT MEMBRANE RES & BIOPHYS, IL-76100 REHOVOT, ISRAEL
关键词
channel-inducing factor; epithelial transport; potassium homeostasis; kidney collecting duct; distal colon;
D O I
10.1152/ajprenal.1997.272.5.F617
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The channel-inducing factor (CHIF) is an epithelial-specific transmembrane protein, which is induced by aldosterone in distal colon (but not in kidney) and can evoke K+ conductance in Xenopus oocytes. The current study examined the possibility that CHIF participates in maintaining K+ balance by assessing its regulation during variations in K+ intake. In adrenal-intact rats, high-K+ diet stimulated, whereas K+ deficiency downregulated, CHIF mRNA both in kidney and colon. The downregulation of CHIF observed in rats fed a low-K+ diet for different periods of time closely correlated with a decrease in plasma K+ but also with changes in aldosterone levels. To differentiate between the two, modulation of CHIF has been studied in adrenalectomized rats with and without corticosteroid supplementation. These experiments have demonstrated that a low-K+ intake suppresses CHIF mRNA, irrespective of aldosterone level. On the other hand, the upregulation evoked by a high-K+ load is apparent only in adrenal-intact rats. This is despite the fact that infusing rats with aldosterone and corticosterone does not increase the expression of this mRNA in kidney. These findings may suggest a role for CHIF in preserving K+ balance.
引用
收藏
页码:F617 / F623
页数:7
相关论文
共 32 条
[1]   A CORTICOSTEROID-INDUCED GENE EXPRESSING AN ISK-LIKE K+ CHANNEL ACTIVITY IN XENOPUS OOCYTES [J].
ATTALI, B ;
LATTER, H ;
RACHAMIM, N ;
GARTY, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (13) :6092-6096
[2]   Cytoplasmic and extracellular IsK peptides activate endogenous K+ and Cl- channels in Xenopus oocytes - Evidence for regulatory function [J].
BenEfraim, I ;
Shai, Y ;
Attali, B .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (15) :8768-8771
[3]   EFFECTS OF CORTICOSTEROID HORMONES ON THE ELECTROPHYSIOLOGY OF RAT DISTAL COLON - IMPLICATIONS FOR NA+ AND K+ TRANSPORT [J].
BINDER, HJ ;
MCGLONE, F ;
SANDLE, GI .
JOURNAL OF PHYSIOLOGY-LONDON, 1989, 410 :425-441
[4]   Cellular localization and regulation of CHIF in kidney and colon [J].
Capurro, C ;
Coutry, N ;
Bonvalet, JP ;
Escoubet, B ;
Garty, H ;
Farman, N .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1996, 271 (03) :C753-C762
[5]  
CAYANIS E, 1992, J MEMBRANE BIOL, V125, P163
[6]   REGULATION OF COLLECTING TUBULE ADENOSINE TRIPHOSPHATASES BY ALDOSTERONE AND POTASSIUM [J].
EIAMONG, S ;
KURTZMAN, NA ;
SABATINI, S .
JOURNAL OF CLINICAL INVESTIGATION, 1993, 91 (06) :2385-2392
[7]   DIETARY POTASSIUM MODULATES ACTIVE POTASSIUM ABSORPTION AND SECRETION IN RAT DISTAL COLON [J].
FOSTER, ES ;
SANDLE, GI ;
HAYSLETT, JP ;
BINDER, HJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1986, 251 (05) :G619-G626
[8]   Differential regulation of putative K+-ATPase by low-K+ diet and corticosteroids in rat distal colon and kidney [J].
Jaisser, F ;
Escoubet, B ;
Coutry, N ;
Eugene, E ;
Bonvalet, JP ;
Farman, N .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1996, 270 (02) :C679-C687
[9]   COMPARISON OF ALDOSTERONE-INDUCED AND RU-28362-INDUCED APICAL NA+ AND K+ CONDUCTANCES IN RAT DISTAL COLON [J].
LOMAX, RB ;
SANDLE, GI .
AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 267 (03) :G485-G493
[10]   MOLECULAR-CLONING AND IMMUNOLOGICAL CHARACTERIZATION OF THE GAMMA-POLYPEPTIDE, A SMALL PROTEIN ASSOCIATED WITH THE NA,K-ATPASE [J].
MERCER, RW ;
BIEMESDERFER, D ;
BLISS, DP ;
COLLINS, JH ;
FORBUSH, B .
JOURNAL OF CELL BIOLOGY, 1993, 121 (03) :579-586