SARS-CoV-2 Infection Dysregulates the Metabolomic and Lipidomic Profiles of Serum

被引：179

作者：

Bruzzone, Chiara ^{[1
]}

Bizkarguenaga, Maider ^{[1
]}

Gil-Redondo, Ruben ^{[1
]}

Diercks, Tammo ^{[2
]}

Arana, Eunate ^{[3
]}

Garcia de Vicuna, Aitor ^{[3
]}

Seco, Marisa ^{[4
]}

Bosch, Alexandre ^{[5
]}

Palazon, Asis ^{[5
]}

San Juan, Itxaso ^{[1
]}

Lain, Ana ^{[1
]}

Gil-Martinez, Jon ^{[1
]}

Bernardo-Seisdedos, Ganeko ^{[6
]}

Fernandez-Ramos, David ^{[1
,7
]}

Lopitz-Otsoa, Fernando ^{[1
]}

Embade, Nieves ^{[1
]}

Lu, Shelly ^{[8
]}

Mato, Jose M. ^{[1
]}

Millet, Oscar ^{[1
,6
]}

机构：

[1] CIC BioGUNE, Basque Res & Technol Alliance, Precis Med & Metab Lab, Derio 48160, Spain

[2] CIC BioGUNE, Basque Res & Technol Alliance, NMR Platform, Derio, Spain

[3] Cruces Univ Hosp, Biocruces Bizkaia Hlth Res Inst, Osakidetza 48903, Barakaldo, Spain

[4] OSARTEN Kooperat Elkartea, Arrasate Mondragon 20500, Spain

[5] CIC BioGUNE, Canc Immunol & Immunotherapy Lab, Basque Res & Technol Alliance, Derio 48160, Spain

[6] ATLAS Mol Pharma SL, Derio 48160, Spain

[7] Inst Salud Carlos III, CIBERehd, Madrid, Spain

[8] Cedars Sinai Med Ctr, Dept Med, Div Digest & Liver Dis, Los Angeles, CA 90048 USA

来源：

ISCIENCE | 2020年 / 23卷 / 10期

关键词：

COVID-19; PLASMA;

D O I：

10.1016/j.isci.2020.101645

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

COVID-19 is a systemic infection that exerts significant impact on the metabolism. Yet, there is little information on how SARS-CoV-2 affects metabolism. Using NMR spectroscopy, we measured the metabolomic and lipidomic serum profile from 263 (training cohort) + 135 (validation cohort) symptomatic patients hospitalized after positive PCR testing for SARS-CoV-2 infection. We also established the profiles of 280 persons collected before the coronavirus pandemic started. Principal-component analysis discriminated both cohorts, highlighting the impact that the infection has on overallmetabolism. The lipidomic analysis unraveled a pathogenic redistribution of the lipoprotein particle size and composition to increase the atherosclerotic risk. In turn, metabolomic analysis reveals abnormally high levels of ketone bodies (acetoacetic acid, 3-hydroxybutyric acid, and acetone) and 2- hydroxybutyric acid, a readout of hepatic glutathione synthesis and marker of oxidative stress. Our results are consistent with a model in which SARS-CoV-2 infection induces liver damage associated with dyslipidemia and oxidative stress.

引用

页数：24

共 33 条

[1]

Thrombosis risk associated with COVID-19 infection. A scoping review [J].

Al-Ani, Fatimah ;

Chehade, Samer ;

Lazo-Langner, Alejro .

THROMBOSIS RESEARCH, 2020, 192 :152-160

[2]

CHAPMAN MJ, 1981, J LIPID RES, V22, P339

[3]

COVID-19 and its implications for thrombosis and anticoagulation [J].

Connors, Jean M. ;

Levy, Jerrold H. .

BLOOD, 2020, 135 (23) :2033-2040

[4]

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (Publication with Expression of Concern) [J].

Corman, Victor M. ;

Landt, Olfert ;

Kaiser, Marco ;

Molenkamp, Richard ;

Meijer, Adam ;

Chu, Daniel K. W. ;

Bleicker, Tobias ;

Bruenink, Sebastian ;

Schneider, Julia ;

Schmidt, Marie Luisa ;

Mulders, Daphne G. J. C. ;

Haagmans, Bart L. ;

van der Veer, Bas ;

van den Brink, Sharon ;

Wijsman, Lisa ;

Goderski, Gabriel ;

Romette, Jean-Louis ;

Ellis, Joanna ;

Zambon, Maria ;

Peiris, Malik ;

Goossens, Herman ;

Reusken, Chantal ;

Koopmans, Marion P. G. ;

Drosten, Christian .

EUROSURVEILLANCE, 2020, 25 (03) :23-30

[5]

Longitudinal plasma metabolomics of aging and sex [J].

Darst, Burcu F. ;

Koscik, Rebecca L. ;

Hogan, Kirk J. ;

Johnson, Sterling C. ;

Engelman, Corinne D. .

AGING-US, 2019, 11 (04) :1262-1282

[6]

Precision High-Throughput Proton NMR Spectroscopy of Human Urine, Serum, and Plasma for Large-Scale Metabolic Phenotyping [J].

Dona, Anthony C. ;

Jimenez, Beatriz ;

Schaefer, Hartmut ;

Humpfer, Eberhard ;

Spraul, Manfred ;

Lewis, Matthew R. ;

Pearce, Jake T. M. ;

Holmes, Elaine ;

Lindon, John C. ;

Nicholson, Jeremy K. .

ANALYTICAL CHEMISTRY, 2014, 86 (19) :9887-9894

[7]

Metabolic Characterization of Advanced Liver Fibrosis in HCV Patients as Studied by Serum 1H-NMR Spectroscopy [J].

Embade, Nieves ;

Marino, Zoe ;

Diercks, Tammo ;

Cano, Ainara ;

Lens, Sabela ;

Cabrera, Diana ;

Navasa, Miquel ;

Falcon-Perez, Juan M. ;

Caballeria, Joan ;

Castro, Azucena ;

Bosch, Jaume ;

Mato, Jose M. ;

Millet, Oscar .

PLOS ONE, 2016, 11 (05)

[8]

Ester M., 1996, KDD-96 Proceedings. Second International Conference on Knowledge Discovery and Data Mining, P226

[9]

Repeated double cross validation [J].

Filzmoser, Peter ;

Liebmann, Bettina ;

Varmuza, Kurt .

JOURNAL OF CHEMOMETRICS, 2009, 23 (3-4) :160-171

[10]

Quantitative Lipoprotein Subclass and Low Molecular Weight Metabolite Analysis in Human Serum and Plasma by 1H NMR Spectroscopy in a Multilaboratory Trial [J].

Jimenez, Beatriz ;

Holmes, Elaine ;

Heude, Clement ;

Tolson, Rose F. ;

Harvey, Nikita ;

Lodge, Samantha L. ;

Chetwynd, Andrew J. ;

Cannet, Claire ;

Fang, Fang ;

Pearce, Jake T. M. ;

Lewis, Matthew R. ;

Viant, Mark R. ;

Lindon, John C. ;

Spraul, Manfred ;

Schaefer, Hartmut ;

Nicholson, Jeremy K. .

ANALYTICAL CHEMISTRY, 2018, 90 (20) :11962-11971

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