Development, characterization, and diagnostic applications of monoclonal antibodies against bovine rotavirus

被引：10

作者：

Al-Yousif, Y ^{[1
]}

Al-Majhdi, F ^{[1
]}

Chard-Bergstrom, C ^{[1
]}

Anderson, J ^{[1
]}

Kapil, S ^{[1
]}

机构：

[1] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2000年 / 7卷 / 02期

关键词：

D O I：

10.1128/CDLI.7.2.288-292.2000

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Hybridomas secreting monoclonal antibodies (MAbs) against the Nebraska calf diarrhea strain of bovine rotavirus (BRV) were characterized. Indirect fluorescent-antibody assay, immunodot assay, and immunoprecipitation were used to select hybridomas that produced anti-BRV MAbs. Seven of the MAbs were shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot assay to be reactive with the BRV outer capsid protein, VP7, which has a molecular mass of 37.5 kDa, None of the seven MAbs were reactive with canine rotavirus, bovine coronavirus, or uninfected Madin-Darby bovine kidney cells. Two clones, 8B4 (immunoglobulin G2a [IgG2a]) and 2B11 (IgG1), were found suitable for use in an antigen capture enzyme-linked immunosorbent assay for detecting BRV in bovine fecal samples. Both were subtype A specific (G6 subtype) but did not react with all isolates of BRV group A.

引用

页码：288 / +

页数：6

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