Directed evolution of new catalytic activity using the α/β-barrel scaffold (Retraction of vol 403, pg 617, 2000)

被引：10

作者：

Altamirano, MM

Blackburn, JM

Aguayo, C

Fersht, AR

机构：

来源：

NATURE | 2002年 / 417卷 / 6887期

关键词：

D O I：

10.1038/417468a

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nature 403, 617–622 (2000). This laboratory reported the in vitro evolution of an enzyme with phosphoribosyl anthranilate isomerase activity (ivePRAI) from the α/β-barrel scaffold of indole-3-glycerol-phosphate synthase using a combination of rationally designed libraries, DNA shuffling, and selection with Escherichia coli, JA300, a strain that lacks an active PRAI gene.

引用

页码：468 / 468

页数：1