PCR-restriction fragment length polymorphism analysis of genes coding for 16S rRNA in Veillonella spp.

被引：26

作者：

Sato, T ^{[1
]}

Sato, M ^{[1
]}

Matsuyama, J ^{[1
]}

Hoshino, E ^{[1
]}

机构：

[1] NIIGATA UNIV,SCH DENT,DEPT PEDIAT DENT,NIIGATA 951,JAPAN

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1997年 / 47卷 / 04期

关键词：

D O I：

10.1099/00207713-47-4-1268

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Restriction fragment length pollymorphism analysis of PCR-amplified 16S ribosomal DNA (16S rDNA PCR-RFLP) was used to generate restriction profiles of the American Type Culture Collection type strains of the genus Veillonella, i.e., V. atypica, V. caviae, V. criceti, V. dispar, V. parvula, V. ratti, and V. rodentium. Whole-cell protein profiles were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for comparative purposes. The 16S rRNA genes were amplified by PCR, and RFLP analysis of the 16S rDNA was performed with MnlI and Sau3AI. MnlI produced six RFLP patterns for seven type strains, since the patterns for V. atypitca and V. caviae were the same, RFLP patterns with Sau3AI could distinguish between V. atypica and V. caviae. The type strains of Veillonella species were easily distinguished by 16S rDNA PCR-RFLP.

引用

页码：1268 / 1270

页数：3

共 14 条

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