Detection of GroEL in activated sludge: a model for detection of system stress

被引：15

作者：

Duncan, AJ

Bott, CB

Terlesky, KC

Love, NG

机构：

[1] Virginia Polytech Inst & State Univ, Dept Civil & Environm Engn, Blacksburg, VA 24061 USA

[2] Fralin Biotechnol Ctr, Blacksburg, VA USA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2000年 / 30卷 / 01期

关键词：

D O I：

10.1046/j.1472-765x.2000.00641.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

GroEL is a ubiquitous constitutively synthesized protein that is also stress inducible. Activated sludge, which is a standard biological process used in wastewater treatment systems, is made up of a diverse microbial consortium. The synthesis of GroEL in activated sludge was significantly induced after heat (42 degrees C) shock. The increased level of GroEL expression was shown to be due to de novo protein synthesis. We have demonstrated a method which shows that stress proteins can be detected in activated sludge, and propose their use as specific indicators of system stress.

引用

收藏

页码：28 / 32

页数：5

相关论文

共 25 条

[1] A panel of stress-responsive luminous bacteria for the detection of selected classes of toxicants [J].

Belkin, S ;

Smulski, DR ;

Dadon, S ;

Vollmer, AC ;

Van Dyk, TK ;

Larossa, RA .

WATER RESEARCH, 1997, 31 (12) :3009-3016

[2] Fate of selected RCRA compounds in a pilot-scale activated sludge system [J].

Bhattacharya, SK ;

Madura, RL ;

Dobbs, RA ;

Angara, RVR ;

Tabak, H .

WATER ENVIRONMENT RESEARCH, 1996, 68 (03) :260-269

[3] UNIQUE AND OVERLAPPING POLLUTANT STRESS PROTEINS OF ESCHERICHIA-COLI [J].

BLOM, A ;

HARDER, W ;

MATIN, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (01) :331-334

[4]

BURLAGE RS, 1997, MANUAL ENV MICROBIOL, P115

[5] AQUATIC HAZARD ASSESSMENT OF THE TOXIC FRACTION FROM THE EFFLUENT OF A PETROCHEMICAL PLANT [J].

DORN, PB ;

VANCOMPERNOLLE, R ;

MEYER, CL ;

CROSSLAND, NO .

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 1991, 10 (05) :691-703

[6] OPTICAL BIOSENSOR FOR ENVIRONMENTAL ONLINE MONITORING OF NAPHTHALENE AND SALICYLATE BIOAVAILABILITY WITH AN IMMOBILIZED BIOLUMINESCENT CATABOLIC REPORTER BACTERIUM [J].

HEITZER, A ;

MALACHOWSKY, K ;

THONNARD, JE ;

BIENKOWSKI, PR ;

WHITE, DC ;

SAYLER, GS .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (05) :1487-1494

[7]

HUBER S, 1997, P 2 INT C MICR ACT S, P645

[8] Induction of heat shock proteins DnaK, GroEL, and GroES by salt stress is Lactococcus lactis [J].

Kilstrup, M ;

Jacobsen, S ;

Hammer, K ;

Vogensen, FK .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (05) :1826-1837

[9]

LITTLE CD, 1991, ON-SITE BIORECLAMATION, P493

[10] 2-DIMENSIONAL GEL-ELECTROPHORESIS ANALYSIS OF THE RESPONSE OF PSEUDOMONAS-PUTIDA KT2442 TO 2-CHLOROPHENOL [J].

LUPI, CG ;

COLANGELO, T ;

MASON, CA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (08) :2863-2872