Production, structure elucidation and anticancer properties of sophorolipid from Wickerhamiella domercqiae

被引：122

作者：

Jing, Chen ^{[1
]}

Xin, Song ^{[1
]}

Hui, Zhang ^{[1
]}

Qu, Yinbo ^{[1
]}

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 39卷 / 03期

关键词：

sophorolipid; Wickerhamiella domercqiae; human cancer cell; anticancer activity;

D O I：

10.1016/j.enzmictec.2005.12.022

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A yeast strain Y-2A producing a large amount of biosurfactants was isolated from oil-containing wastewater and identified as Wickerhamiella domercqiae by BIOLOG analysis and routine yeast identification method. The crude biosurfactants produced from Y-2A were obtained by extract with ethyl acetate and proved to be a mixture of glycolipids by thin layer chromatography (TLC). The main product was separated and purified by HPLC and then characterized as sophorolipid by nuclear magnetic resonance (NMR) and mass spectroscopy (MS). This is the first report on sophorolipids produced from W. domercqiae. Subsequently, the cytotoxic effects of the sophorolipid on cancer cells of H7402, A549, HL60 and K562 were investigated by MTT assay. The results showed a dose-dependent inhibition ratio on cell viability according to the drug concentration <= 62.5 mu g/ml. These findings suggested that the sophorolipid produced by W domercqiae have anticancer activity. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：501 / 506

页数：6

共 18 条

[1]

MICROBIAL-PRODUCTION, STRUCTURE ELUCIDATION AND BIOCONVERSION OF SOPHOROSE LIPIDS [J].