Both the conserved GRAS domain and nuclear localization are required for SHORT-ROOT movement

被引:107
作者
Gallagher, Kimberly L.
Benfey, Philip N. [1 ,2 ]
机构
[1] Duke Univ, Dept Biol, Durham, NC 27708 USA
[2] Duke Univ, IGSP Ctr Syst Biol, Durham, NC 27708 USA
关键词
SHORT ROOT; Arabidopsis root development; intercellular protein movement; plasmodesmata; protein trafficking; CELL-TO-CELL; GREEN FLUORESCENT PROTEIN; TRANSCRIPTION FACTOR EXPRESSION; ARABIDOPSIS ROOT; INTERCELLULAR MOVEMENT; HOMEOTIC PROTEINS; MESSENGER-RNA; TRAFFICKING; PLASMODESMATA; PLANTS;
D O I
10.1111/j.1365-313X.2008.03735.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transcription factor movement is well established in plants. Since the initial report of KNOTTED movement, more than a dozen transcription factors have been shown to move in plants. However, the developmental significance of movement is not known. Using the SHORT-ROOT (SHR) transcription factor as a tool for studying cell-to-cell trafficking, we show that movement of SHR from its site of synthesis is necessary for normal development of the Arabidopsis root. We identify multiple regions of SHR that are required for intra- and intercellular movement of SHR, including a region that is necessary for movement but not activity. We made the surprising discovery that the capacity for intercellular movement may be conserved among other GRAS family proteins. Finally, we provide evidence that movement requires both cytoplasmic and nuclear localization, strongly suggesting a mechanistic link between nuclear transport and cell-to-cell movement.
引用
收藏
页码:785 / 797
页数:13
相关论文
共 46 条
[1]   A subclass of plant heat shock cognate 70 chaperones carries a motif that facilitates trafficking through plasmodesmata [J].
Aoki, K ;
Kragler, F ;
Xoconostle-Cázares, B ;
Lucas, WJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (25) :16342-16347
[2]   Primer - Root development [J].
Benfey, PN ;
Scheres, B .
CURRENT BIOLOGY, 2000, 10 (22) :R813-R815
[3]   The taming of the cell penetrating domain ofthe HIV Tat: Myths and realities [J].
Chauhan, Ashok ;
Tikoo, Akshay ;
Kapur, Arvinder K. ;
Singh, Mahavir .
JOURNAL OF CONTROLLED RELEASE, 2007, 117 (02) :148-162
[4]   Non-targeted and targeted protein movement through plasmodesmata in leaves in different developmental and physiological states [J].
Crawford, KM ;
Zambryski, PC .
PLANT PHYSIOLOGY, 2001, 125 (04) :1802-1812
[5]   An evolutionarily conserved mechanism delimiting SHR movement defines a single layer of endodermis in plants [J].
Cui, Hongchang ;
Levesque, Mitchell P. ;
Vernoux, Teva ;
Jung, Jee W. ;
Paquette, Alice J. ;
Gallagher, Kimberly L. ;
Wang, Jean Y. ;
Blilou, Ikram ;
Scheres, Ben ;
Benfey, Philip N. .
SCIENCE, 2007, 316 (5823) :421-425
[6]   Mechanisms regulating SHORT-ROOT intercellular movement [J].
Gallagher, KL ;
Paquette, AJ ;
Nakajima, K ;
Benfey, PN .
CURRENT BIOLOGY, 2004, 14 (20) :1847-1851
[7]  
Gisel A, 1999, DEVELOPMENT, V126, P1879
[8]   PLASMODESMATA AND CELL-TO-CELL TRANSPORT IN PLANTS [J].
GUNNING, BES ;
OVERALL, RL .
BIOSCIENCE, 1983, 33 (04) :260-265
[9]   Nuclear export of proteins in plants:: AtXPO1 is the export receptor for leucine-rich nuclear export signals in Arabidopsis thaliana [J].
Haasen, D ;
Köhler, C ;
Neuhaus, G ;
Merkle, T .
PLANT JOURNAL, 1999, 20 (06) :695-705
[10]   Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly [J].
Haseloff, J ;
Siemering, KR ;
Prasher, DC ;
Hodge, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2122-2127