On-line measurement of green fluorescent protein (GFP) fluorescence for the monitoring of recombinant adenovirus production

被引：25

作者：

Gilbert, PA

Garnier, A

Jacob, D

Kamen, A ^{[1
]}

机构：

[1] Univ Laval, Fac Sci & Genie, Dept Genie Chim, St Foy, PQ G1K 7P4, Canada

[2] Natl Res Council Canada, Biotechnol Res Inst, Bioproc Sector, Montreal, PQ H4P 2R2, Canada

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

GFP; mammalian cell culture; on-line fluorescence monitoring; recombinant adenovirus production;

D O I：

10.1023/A:1005672410905

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An on-line fluorescence sensor prototype was constructed to monitor the production of the green fluorescent protein (GFP) by 293S cells infected with a recombinant adenovirus vector (rAdV) containing the GFP gene. Fluorescence was correlated to GFP concentration and therefore to viral protein expression, but not to rAdV production. The sensor signal can also be used to compute the GFP production rate, which predicts well the occurrence of maximum viral titer.

引用

页码：561 / 567

页数：7

共 17 条

[1] Green fluorescent protein as a real time quantitative reporter of heterologous protein production [J].