Simultaneous determination of bisphenol A and its halogenated derivatives in river water by combination of isotope imprinting and liquid chromatography-mass spectrometry

A restricted access media-molecularly imprinted polymer (RAM-MIP) for [H-2(16)]bisphenol A (BPA-d(16)) was prepared by a multi-step swelling and polymerization method using 4-vinylpyridine as a functional monomer and ethylene glycol dimethacrylate as a cross-linker, followed by hydrophilic surface modification using glycerol dimethacrylate and glycerol monomethacrylate as hydrophilic monomers. The obtained RAM-MIP showed excellent molecular recognition abilities for BPA and BFA-d(6) as well as BPA-d16 used as the template molecule, and good ones for tetrachlorobisphenol A (Cl-4-BPA) and tetrabromobisphenol A (Br-4-BPA). Next, the RAM-MIP was utilized for selective on-line pretreatment and enrichment of BPA, Cl-4-BPA and Br-4-BPA in a river water sample, followed by their separation and determination by LC-MS. The calibration graphs of BPA, Cl-4-BPA and Br-4-BPA, constructed using BPA-d(6) as an internal standard, showed good linearity in the range of 12.5-200 pg/mL (r > 0.999) with a 2-mL injection of a river water sample. The inter-day precision data for the assay of BPA, Cl-4-BPA and Br-4-BPA at 25 pg/mL were 1.08, 3.67 and 1.58%, respectively. Furthermore, this method was successfully applied for the simultaneous determination of BPA and its halogenated derivatives in river water. (c) 2006 Elsevier B.V. All rights reserved.