FHA domain boundaries of the Dun1p and Rad53p cell cycle checkpoint kinases

被引：31

作者：

Hammet, A ^{[1
]}

Pike, BL ^{[1
]}

Mitchelhill, KI ^{[1
]}

Teh, T ^{[1
]}

Kobe, B ^{[1
]}

House, CM ^{[1
]}

Kemp, BE ^{[1
]}

Heierhorst, J ^{[1
]}

机构：

[1] St Vincents Inst Med Res, Fitzroy, Vic 3065, Australia

来源：

FEBS LETTERS | 2000年 / 471卷 / 2-3期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

forkhead-associated domain; Dun1p; Rad53p; Chk2; protein kinase; cell cycle;

D O I：

10.1016/S0014-5793(00)01392-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by sequence comparisons (55-75 residues), In vivo, expression of the proteolytically defined Dun1p FHA domain, but not a fragment containing only the predicted domain boundaries, inhibited the transcriptional induction of repair genes following replication blocks, This indicates that the non-catalytic FI-IA domain plays an important role in the transcriptional function of the Dun1p protein kinase. (C) 2000 Federation of European Biochemical Societies.

引用

页码：141 / 146

页数：6

共 29 条

[1] THE SAD1/RAD53 PROTEIN-KINASE CONTROLS MULTIPLE CHECKPOINTS AND DNA DAMAGE-INDUCED TRANSCRIPTION IN YEAST [J].

ALLEN, JB ;

ZHOU, Z ;

SIEDE, W ;

FRIEDBERG, EC ;

ELLEDGE, SJ .

GENES & DEVELOPMENT, 1994, 8 (20) :2401-2415

[2] Heterozygous germ line hCHK2 mutations in Li-Fraumeni syndrome [J].

Bell, DW ;

Varley, JM ;

Szydlo, TE ;

Kang, DH ;

Wahrer, DCR ;

Shannon, KE ;

Lubratovich, M ;

Verselis, SJ ;

Isselbacher, KJ ;

Fraumeni, JF ;

Birch, JM ;

Li, FP ;

Garber, JE ;

Haber, DA .

SCIENCE, 1999, 286 (5449) :2528-2531

[3] A human Cds1-related kinase that functions downstream of ATM protein in the cellular response to DNA damage [J].

Brown, AL ;

Lee, CH ;

Schwarz, JK ;

Mitiku, N ;

Piwnica-Worms, H ;

Chung, JH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) :3745-3750

[4] Red1p, a MEK1-dependent phosphoprotein that physically interacts with Hop1p during meiosis in yeast [J].

de los Santos, T ;

Hollingsworth, NM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (03) :1783-1790

[5] The FHA domain is a modular phosphopeptide recognition motif [J].

Durocher, D ;

Henckel, J ;

Fersht, AR ;

Jackson, SP .

MOLECULAR CELL, 1999, 4 (03) :387-394

[6] Cell cycle checkpoints: Preventing an identity crisis [J].

Elledge, SJ .

SCIENCE, 1996, 274 (5293) :1664-1672

[7] RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast [J].

Gardner, R ;

Putnam, CW ;

Weinert, T .

EMBO JOURNAL, 1999, 18 (11) :3173-3185

[8] Ca2+/S100 regulation of giant protein kinases [J].

Heierhorst, J ;

Kobe, B ;

Feil, SC ;

Parker, MW ;

Benian, GM ;

Weiss, KR ;

Kemp, BE .

NATURE, 1996, 380 (6575) :636-639

[9] THE FHA DOMAIN - A PUTATIVE NUCLEAR SIGNALING DOMAIN FOUND IN PROTEIN-KINASES AND TRANSCRIPTION FACTORS [J].

HOFMANN, K ;

BUCHER, P .

TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (09) :347-349

[10] The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor [J].

Huang, MX ;

Zhou, Z ;

Elledge, SJ .

CELL, 1998, 94 (05) :595-605

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