Conjugative transposon Tn916: Evidence for excision with formation of 5'-protruding termini

被引：17

作者：

Manganelli, R

Ricci, S

Pozzi, G

机构：

[1] UNIV SIENA,DIPARTIMENTO BIOL MOL,SEZ MICROBIOL,I-53100 SIENA,ITALY

[2] UNIV CAGLIARI,DIPARTIMENTO SCI CHIRURG,SEZ MICROBIOL,CAGLIARI,ITALY

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 19期

关键词：

D O I：

10.1128/jb.178.19.5813-5816.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Conjugative transposons are genetic elements able to promote their own intracellular transposition and intercellular conjugal transfer, They move by an excision-integration system related to that of lambdoid phages, in which the first step is the excision of the transposon from the donor replicon to form a covalently closed circular intermediate which contains a heteroduplex joint, In this work, sequencing both strands of the circular intermediate heteroduplex joint, it was found that, as during lambda phage excision, Tn916 excises from the host DNA by 5'-protruding staggered endonucleolytic cleavages.

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页码：5813 / 5816

页数：4

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